Figures index : Rutin Attenuates Lipopolysaccharide-induced Nitric Oxide Production in Macrophage Cells : Science and Education Publishing

Figures index

Journal of Food and Nutrition Research. 2015, 3(3), 202-205 doi:10.12691/jfnr-3-3-12

Figure 1. Effect of rutin and/or LPS on the viability and NO production of RAW264.7 cells examined using the MTT and NO assays. A: Chemical structure of rutin. B: RAW264.7 cells were incubated with 0, 0.1, 1, 10, 30, 50 and 100 μM of rutin for 24 h. C: RAW264.7 cells were pretreated for 4 h with rutin, and then with LPS (100 ng/mL) for 18 h. The concentration of NO in the culture medium was determined using the Griess assay. The results are shown as percentages of control samples. Data are presented as the means ± SEM for three independent experiments (n=3). Significance was determined by Student’s t-test. *(p < 0.05), **(p < 0.01), and ***(p < 0.001). #Not significantly different from the LPS-treated group (p > 0.05)

Figure 2. Effect of rutin on LPS-induced expression of COX-2 (A) and iNOS (B). RAW264.7 cells were pretreated with 30 μM of rutin and then exposed to LPS (100 ng/mL) for 12 h. Total cell lysates (30 μg protein) were separated on 10% SDS-PAGE. β-actin was used as the loading control. Proteins were detected by western blotting (upper panels). Protein expression was quantitated by densitometric analysis of the western blots, and is shown in the lower panels (n=3). Significantly different compared with the LPS-stimulated group (p < 0.05 by the paired t-test)