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From
Potential Genitourinary Toxicity and Lithogenic Effect of Ractopamine
Wen-Chi Chen, Yu-Chi Wang, Jui-Lung Shen, Huey-Yi Chen, Chiao-Hui Chang, Fuu-Jen Tsai, Wei-Yong Lin, Yung-Hsiang Chen
Journal of Food and Nutrition Research
.
2015
, 3(10), 670-674 doi:10.12691/jfnr-3-10-9
Fig
ure
1.
Effect of ractopamine on cell viability in (
A
) HK-2, MBT-2, MDCK, (
B
) SV40 MES 13, and SV-HUC-1 cells. Cells were incubated with medium alone (control) or various concentrations of ractopamine for 24 h, and the cell viability was measured using the MTT assay. Data are means ± S.D. from 3 independent experiments. *
P
< 0.05 vs. control condition
Full size figure and legend
Fig
ure
2
. EG- and ractopamine-induced crystal deposition in the Malpighian tubules of
Drosophila
. (
A
) The images show representative polarized microscopy for the flies with 0.5% EG- and 10 ppb ractopamine-induced crystal formation in Malpighian tubules. (
B
) Rates of crystal formation in control, 0.5% EG-, and 10 ppb ractopamine-treated
Drosophila
(n ≅ 150 for each group). **
P
< 0.001, *
P
< 0.05, compared to the control group
Full size figure and legend
Fig
ure
3.
Ractopamine inhibited geotaxis climbing behavior. Climbing behavior were measured in ractopamine-treated flies on day 21. Ractopamine induced a decrease in fly geotaxis climbing ability. Climbing ability was impaired at non-lethal ractopamine concentration on day 21. *
P
< 0.05, compared to the control group
Full size figure and legend
Fig
ure
4
.
Lifespan of control and ractopamine-treated flies. Cumulative survival distributions by administration of 10 ppb ractopamine. Flies treated with ractopamine showed significant life-span reduction compared with control group (
n
≅ 150 for each group,
P
< 0.05 from log-rank test)
Full size figure and legend