Figures index

From

High Anti-oxidative and Lipid-lowering Activities of Flavonoid Glycosides-rich Extract from the Leaves of Zanthoxylum bungeanum in Multi-system

Limei Ma, Ke Li, Dandan Wei, Hengyi Xiao, Hai Niu, Wen Huang

Journal of Food and Nutrition Research. 2015, 3(1), 62-68 doi:10.12691/jfnr-3-1-11
  • Figure 1. DPPH radical scavenging activity of ZLE. The absorbance of the DPPH at 510 nm treated with different concentrations of ZLE (A) and a microplate showed the reaction of DPPH with different concentrations of ZLE (B), 2.5 μg/mL (dark purple), 5 μg /mL (pink purple),10 μg /mL (yellowish), 20 μg/mL (light yellowish)
  • Figure 2. ZLE had no detected cytotoxic effects on HepG2 cells. Cell morphology treated with different concentration of ZLE for 24 h (A) andCell viability of ZLE was detected by MTT assay (B). ZLE had no detected inhibition on proliferation of HepG2 cells in concentration of 100-1000 μg/mL. Values are presented as mean ± SE of three experiments. P < 0.05, vs control group
  • Figure 3. ZLE decreased the lipid accumulation induced by FFAs in a dose-dependent manner. (A) Control group: cells treated with DMEM for 24 h; (B) FFAs group: cells treated with 1 mM FFAs for 24 h; (C) Cells treated with fenofibrate in a dose of 100 μM for 24 h; (D,E,F) Cells were treated with ZLE in a various concentration of 125, 250, 500 μg /mL for 24 h. The cells were stained with Oil red O for measuring intracellular lipid accumulation and examined by light microscopy. Amicroplate showed the lipid content in different groups (G., H); Results are representative of 3 independent experiments (n=3)
  • Figure 4. ZLE decreased the lipid levels in a dose-dependent manner. The triglyceride and cholesterol levels in HepG2 cells were measured with a Colorimetric/Fluorometric Kit; Results are representative of 3 independent experiments (n=3), *p < 0.05 vs FFAs group. The effect of ZLE on serum lipids in apoE-/- mice were showed in Fig 4 Ⅱ. Group A: Control group, use 0.5% CMC; Group B: Model group given 0.5% CMC fed with a high diet. Group C: ZLE group given a dosage of 250 mg/(kg.d); and Group D are positive group given a dosage of fenofibrate (100 mg/kg.d). The experiment last 2 months, lipid levels in model group were significantly higher than those in the control group, and treatment with ZLE (250 mg/kg.d) had great effect on the level of lipids compared with fenofibrate. *p < 0.05 vs Control group; # p < 0.05 vs Model group
  • Figure 5. Effect of ZLE on histology changes in apoE-/- mice. Higstoppathology of liver, kidney and lung from apoE-/- mice (HE staining: original magnification ×20). The mice were treated with 0.5% CMC (A), 250 mg/kg ZLE (B), 100 mg/kg fenofibrate (C) for 8 weeks. The main organs were collected and processed for histological analysis. The sections were stained with hematoxylin-eosin. No detectable injures were examined, suggesting that the ZLE had no distinct toxicity on liver, kidney and lung and well tolerated