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Green Tea Polyphenol Epigallocatechin-3-O-Gallate Attenuates Lipopolysaccharide-induced Nitric Oxide Production in RAW264.7 Cells

Seung-Jae Lee, Hyun Woo Kang, Seung Yuan Lee, Sun Jin Hur

Journal of Food and Nutrition Research. 2014, 2(7), 425-428 doi:10.12691/jfnr-2-7-16
  • Figure 1. Effect of EGCG on cell viability and induced NO production in RAW264.7 cells, with or without LPS-stimulation. (A) Chemical structure of EGCG. (B) Cell viability of RAW264.7 cells incubated with 0.1, 1.0, and 10 μM EGCG and LPS (50 ng/mL) for 24 h determined using MTT assay. (C) NO concentration in the culture medium following treatment with EGCG (1.0 µg/mL) and/or LPS (50 ng/mL) determined using Griess assay. Data are presented as mean ± S.E.M. (n = 3) for three independent experiments. жNot significantly different from that of the control group. #Not significantly different from the LPS-treated group. Significance was determined by Student’s t-test. *p < 0.05
  • Figure 2. Effect of EGCG on LPS-induced iNOS (A) and COX-2 (B) expression. Relative expression of iNOS and COX-2 to control (β-actin) was performed by densitometric analysis of gels (lower). *p < 0.05 and **p < 0.01 are significantly different as analyzed by paired t-test compared with LPS-stimulated group