Figure 3. Effect of aged garlic extract (AGE) on glucose transporter 1 (GLUT1) protein expression in glial fibrillary acidic protein (GFAP) positive astrocytes.A. Representative images of double labeled cells with antibodies against GFAP and GLUT1; nuclei stained with DAPI. B. Anatomical position of cortex analyzed (contralateral and ipsilateral to lesion). C. Representative images from ipsilateral series, cells stained with antibody against GLUT1 and GFAP, nuclei stained with DAPI. Reference bar = 10 m. D. Quantitative analysis of GLUT1 positive astrocytes (GFAP positive) normalized by the number of DAPI stained nuclei and expressed as percentage. Rats were subjected to middle cerebral artery occlusion (MCAO) for 2 h and sacrificed after 2 h of reperfusion. AGE (360 mg/kg; i.p) was injected at the beginning of reperfusion. One way-ANOVA, Tukey, *P<0.05 vs control; &P<0.05 : Glucose Transporter 1 Expression Is Regulated by Aged Garlic Extract during Cerebral Ischemia : Science and Education Publishing

Figure 3. Effect of aged garlic extract (AGE) on glucose transporter 1 (GLUT1) protein expression in glial fibrillary acidic protein (GFAP) positive astrocytes.A. Representative images of double labeled cells with antibodies against GFAP and GLUT1; nuclei stained with DAPI. B. Anatomical position of cortex analyzed (contralateral and ipsilateral to lesion). C. Representative images from ipsilateral series, cells stained with antibody against GLUT1 and GFAP, nuclei stained with DAPI. Reference bar = 10 m. D. Quantitative analysis of GLUT1 positive astrocytes (GFAP positive) normalized by the number of DAPI stained nuclei and expressed as percentage. Rats were subjected to middle cerebral artery occlusion (MCAO) for 2 h and sacrificed after 2 h of reperfusion. AGE (360 mg/kg; i.p) was injected at the beginning of reperfusion. One way-ANOVA, Tukey, *P<0.05 vs control; &P<0.05

Journal of Food and Nutrition Research. 2014, 2(12), 899-905 doi:10.12691/jfnr-2-12-7