Figure 5. The effects of extraction fractions on PC12 cells. (a) The inhibitory of PC12 cells lesion induced by Aβ25-35 after treatment with various concentrations (0.5, 0.2, 0.1, 0.05, 0.01, 0.005 mg/mL) of the fractions demonstrated that the EtOAc fraction could be of the best capacity, followed by n-BuOH, MeOH, H2O and CH2Cl2 fractions. (b) Effect of A. Grossedentata extracts on 10 μM Aβ25-35 induced intracellular ROS generation in PC12 cells. Data showed that PC12 cells exposure to Aβ25-35 resulted in significant levels of ROS generation. The different fractions of A. Grossedentata MeOH extracts inhibited intracellular ROS generation. (c) DPPH radical scavenging activity ofthe fractions was basically identical, and in a dose dependent manner : Study on Neuroprotective Capacity of Key Flavonoids of Ampelopsis grossedentata : Science and Education Publishing

Figure 5. The effects of extraction fractions on PC12 cells. (a) The inhibitory of PC12 cells lesion induced by Aβ_25-35 after treatment with various concentrations (0.5, 0.2, 0.1, 0.05, 0.01, 0.005 mg/mL) of the fractions demonstrated that the EtOAc fraction could be of the best capacity, followed by n-BuOH, MeOH, H₂O and CH₂Cl₂ fractions. (b) Effect of A. Grossedentata extracts on 10 μM Aβ_25-35 induced intracellular ROS generation in PC12 cells. Data showed that PC12 cells exposure to Aβ_25-35 resulted in significant levels of ROS generation. The different fractions of A. Grossedentata MeOH extracts inhibited intracellular ROS generation. (c) DPPH radical scavenging activity ofthe fractions was basically identical, and in a dose dependent manner

Journal of Food and Nutrition Research. 2014, 2(12), 880-889 doi:10.12691/jfnr-2-12-4