Figures index : Analysis of Major Constituents in Seed Cells of Aquilaria sinensis : Science and Education Publishing

Figures index

Journal of Food and Nutrition Research. 2014, 2(1), 34-39 doi:10.12691/jfnr-2-1-6

Figure 1. Electron microscopy of seed cells of Aquilaria sinensis. Protein bodies and oil bodies in a seed cell of Aquilaria sinensis were labeled as PB and OB, respectively. Bar at the bottom represents 2 μm

Figure 2. SDS-PAGE of Aquilaria seed proteins fractionated by centrifugation. Total extract of Aquilaria seed proteins was centrifugated at 10,000 × g to yield three fractions, supernatant (Sup.), pellet and oil bodies (OB). The major protein bands in the three fractions were also indicated by arrows in the total extract. Labels in the left indicate the molecular masses of marker proteins

Figure 3. SDS-PAGE of the major soluble and insoluble proteins of Aquilaria seeds. The major band of the supernatant (10 kDa) and tthat of the pellet (55 kDa) were subjected to SDS-PAGE analysis in the absence and presence of β-mercaptoethanol (β-ME). Putative polypeptides of 2 S albumin and 11 S globulin are indicated

Figure 4. SDS-PAGE of proteins from sesame and Aquilaria oil bodies (A). Proteins extracted from oil bodies of sesame and Aquilaria seeds were resolved by SDS-PAGE. Putative oleosin (17 kDa) and caleosin (27 kDa) indicated by arrows (labeled with b and c) were subjected to mass spectrometric analysis after trypsin digestion (B and C). The peptide fragment identified to be related to oleosin or caleosin was shown on the right top corner. All matched b and y ions were labeled in the figures

Figure 5. Neutral lipids extracted from oil bodies of sesame and Aquilaria seeds analyzed by TLC. The positions of triacylglycerols (TAG) and diacylglycerols (DAG) are indicated in the right margin