Figure 3. Flow cytometric analysis of apoptosis progression in A-549 cells treated with E-OHS. The cells were exposed to various concentrations (0, 0.1% DMSO, 50, 75, or 100 μg/mL) for 12 h. The cells were labeled with monoclonal antibodies specific for Annexin V-FITC and PI. Based on the staining pattern, cells were classified as follows: Annexin−/PI− (LL), indicating viable cells; Annexin+/PI− (LR), indicating cells undergoing early apoptosis, Annexin+/PI+ (UR), indicating late apoptotic cells; Annexin-/PI+ (UL), indicating necrotic cells

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Anti-cancer Effect of the Ethyl Acetate Fraction from Opuntia humifusa on A-549 Human Lung Cancer Cells

Eon Ji Yeo, Myung Chul Cha, Dong Seok Lee

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