Figures index

From

Combined Physicochemical and Transcriptomic Analyses Reveal the Effect of Black Wolfberry Ferment on Alopecia

Ping Yu, Xiangbo Min, Lina Li, Yu Wang, Di Zhao

Journal of Food and Nutrition Research. 2025, 13(5), 195-205 doi:10.12691/jfnr-13-5-1
  • Figure 1. Effect of black wolfberry ferment on androgenetic alopecia in mice. (A) Hair regrowth in areas of testosterone propionate-induced hair removal. (B) The weight of newborn hairs. C: control group; M: model group; LF: low-dosage ferment group; HF: high-dosage ferment group. Student’s t-test was used to compare significant differences, with * representing P < 0.05, ** representing P < 0.01, and *** representing P < 0.001 compared to the M group
  • Figure 2. Histopathology of dorsal skins in the newborn hair area. (A) Masson staining. (B) Collagen fiber area. (C) HE staining. (D) The number of hair follicles. (E) The number of terminal hair and vellus hair. (F) Dermal thickness and hypodermis thickness. C: control group; M: model group; LF: low-dosage ferment group; HF: high-dosage ferment group. Student’s t-test was used to compare significant differences, with * representing P < 0.05, ** representing P < 0.01, and *** representing P < 0.001 compared to the M group
  • Figure 3. Expression of Ki67- and Sox9-positive cells in hair follicles. (A) Immunofluorescence staining image of Ki67. (B) Positive rate of Ki67. (C) Immunofluorescence staining image of Sox9. (D) Positive rate of Sox9. C: control group; M: model group; LF: low-dosage ferment group; HF: high-dosage ferment group. Student’s t-test was used to compare significant differences, with * representing P < 0.05, ** representing P < 0.01, and *** representing P < 0.001 compared to the M group
  • Figure 4. Key modules identified by WGCNA. (A) The scale-free network fitting index for different soft-thresholding powers. (B) The mean connectivity for different soft-thresholding powers. (C) Clustering dendrogram. (D) Eigengenes dendrogram. (E) Eigengenes adjacency heatmap
  • Figure 5. The screening of key genes. (A) Heatmap of the correlation (numbers on the top) and P value (numbers on the bottom) between module and hair regrowth features. (B) The number of DEGs between M group and C, LF, HF groups, respectively. (C) Overlapping results of gene numbers in MEsalmon and DEGs
  • Figure 6. Western blot analysis. (A) The protein expression of PIK3R5, FOXO3,and PTPN7. (B) Corresponding quantification. C: control group; M: model group; LF: low-dosage ferment group; HF: high-dosage ferment group. Student’s t-test was used to compare significant differences, with * representing P < 0.05, ** representing P < 0.01, and *** representing P < 0.001 compared to the M group
  • Figure S1. Profiles of transcriptome data. (A) PCA plot of samples. (B) Overlapping results of gene numbers in each group. (C) Hierarchical clustering tree of 12 samples
  • Figure S2. Functional enrichment analyses for (A) GO functional items and (B) KEGG pathway