Figure 4. Flow cytometry analysis of apoptosis progression in HT-29 cells treated with kaempferol alone, quercetin alone, kaempferol + quercetin, kaempferol + quercetin + OJE, and OJE. The cells were exposed to various concentrations (kaempferol 100 µM, quercetin 100 µM, kaempferol 50 µM + quercetin 50 µM, kaempferol 33 µM + quercetin 33 µM + OJE 33µg/mL, and OJE 100µg/mL) fo 12 h. The cells were labeled with monoclonal antibodies specific to Annexin V-FITC and PI. Based on the staining pattern, cells were categorized as follows: Annexin−/PI− (LL) representing viable cells, Annexin+/PI− (LR) indicating cells undergoing apoptosis, and Annexin+/PI+ (UR) representing cells in end-stage of apoptosis or already deceased : Characterization of Anti-Cancer Effects of the Ethyl Acetate Fraction from Orostachys japonicus on HT-29 Human Colon Cancer Cells : Science and Education Publishing

Figure 4. Flow cytometry analysis of apoptosis progression in HT-29 cells treated with kaempferol alone, quercetin alone, kaempferol + quercetin, kaempferol + quercetin + OJE, and OJE. The cells were exposed to various concentrations (kaempferol 100 µM, quercetin 100 µM, kaempferol 50 µM + quercetin 50 µM, kaempferol 33 µM + quercetin 33 µM + OJE 33µg/mL, and OJE 100µg/mL) fo 12 h. The cells were labeled with monoclonal antibodies specific to Annexin V-FITC and PI. Based on the staining pattern, cells were categorized as follows: Annexin−/PI− (LL) representing viable cells, Annexin+/PI− (LR) indicating cells undergoing apoptosis, and Annexin+/PI+ (UR) representing cells in end-stage of apoptosis or already deceased

Journal of Food and Nutrition Research. 2025, 13(3), 127-139 doi:10.12691/jfnr-13-3-2