Figure 5. ERK1/2 activation by E-OJ and its role in apoptosis in HeLa cells. (A) HeLa cells were pretreated with U0126, a selective ERK1/2 inhibitor, followed by treatment with 10 μg/ml E-OJ for 15 minutes. Phospho-ERK1/2 and total ERK1/2 protein levels were assessed by Western blotting. (B) Cells were treated with 10 μg/ml E-OJ for 12 hours in the presence or absence of U0126, and expression of cleaved caspase-3 and caspase-3 was evaluated. GAPDH was used as a loading control. Densitometric analysis was conducted based on three independent experiments. Data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001

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Orostachys Japonicus Promotes Apoptosis in Cervical Cancer Cells through Modulation of NF-ΚB and ERK1/2 Pathways

Seon-Hee Kim, Dong Seok Lee

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