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From
Endothelium-Dependent Vasorelaxant Effect of a Bioactive Tripeptide, Valine-Proline-Proline, on Rat Aortic Rings
Jair Lozano-Cuenca, Oscar Alberto López-Canales, Ángel Miliar-García, María de los Ángeles Martínez–Godínez, Osiris Teran-Gallardo, María Cristina Paredes-Carbajal, Ruth Mery López-Mayorga, Enrique Fernando Castillo-Henkel, Héctor Flores-Herrera, Jorge Skiold López-Canales
Journal of Food and Nutrition Research
.
2023
, 11(8), 519-524 doi:10.12691/jfnr-11-8-1
Figure 1.
Concentration-dependent relaxation of 10
-9
- 10
-4
M Val-Pro-Pro on endothelium-intact (closed circles) and endothelium-denuded (open circles) phenylephrine-precontracted rat aortic rings. Data are expressed as the mean ± SEM of n observations (n= 6). (*) P<0.001 vs (+) Endothelium.
Full size figure and legend
Figure 2.
Effects of 10
-5
M L-NG-Nitroarginine Methyl Ester (L-NAME) (A) and 10
-2
M tetraethylammonium (TEA) (B) on Val-Pro-Pro-induced vasorelaxation in functional endothelium rat aortic rings precontracted with 10
-6
M phenylephrine (PE). Data are expressed as the mean ± SEM of n observations. (*) P<0.001 vs control.
Full size figure and legend
Figure 3.
Effects of: (A) 10
-7
M apamin plus 10
-7
M charybdotoxin, (B) 3.1 x 10
-7
M glibenclamide and (C) 10
-3
M 4-aminopyridine (4-AP) on Val-Pro-Pro-induced vasorelaxation in functional endothelium rat aortic rings precontracted with 10
-6
M phenylephrine (PE). Data are expressed as the mean ± SEM of n observations (n=6). * P<0.001 vs control (two-way ANOVA).
Full size figure and legend
Figure 4.
Effects of 10
-7
M ODQ (A) and 10
-6
M KT 5823 (B) on Val-Pro-Pro-induced vasorelaxation in functional endothelium rat aortic rings precontracted with 10
-6
M phenylephrine (PE). Data are expressed as the mean ± SEM of n observations. (*) P<0.001 vs control (two-way ANOVA).
Full size figure and legend
Figure 5.
Effects of 10
-5
M Indomethacin (A) and 10
-5
M cycloheximide (B) on Val-Pro-Pro-induced vasorelaxation in functional endothelium rat aortic rings precontracted with 10
-6
M phenylephrine (PE). Data are expressed as the mean ± SEM of n observations.
Full size figure and legend