Figures index

From

Isolation and Cloning of sprA Gene Alkaline Protease Activity from Streptomyces flavogriseus ADEM7 and Characterization of Its Antioxidant and Antitumor Activities

Doaa E. El-Hadedy, Nesreen A. Safwat, Abir Pertila, Rafat A. Siddiqui

Journal of Food and Nutrition Research. 2023, 11(5), 345-352 doi:10.12691/jfnr-11-5-2
  • Figure 1. Restriction map and general specification of the cloning PGEM-T easy vector. The sequence of the multiple cloning sites is shown with a PCR product inserted by TAT cloning strategy
  • Figure 2. The dose-response curve showing the in vitro inhibitory activity of the alkaline protease enzyme from the Streptomyces flavogriseus (exposed to gamma radiation or non-exposed) against human carcinoma cells after 48 h treatment using MTT assay. Where: (A) liver (HepG2) cell line, (B) breast (MCF-7) cell line, (C) colorectal intestinal (CACO2) cell line, (D) colon carcinoma (HCT-116) cell line. Results were shown as the mean viable cells % ± standard deviation of three independent experiments
  • Figure 3. DPPH radical scavenging activity of the alkaline protease enzyme from the Streptomyces flavogriseus (exposed to gamma radiation or non-exposed). Results were shown as the mean radical scavenging percentage ± standard deviation of three independent experiments
  • Figure 4. Diagrams illustrate Insertion SprA gene expressed extracellular protease alkaline fragment in PGEM vector by ligase enzyme