Figure 5. Effects of n-hexane extract of C. oleifera fruit hull on the phosphorylation level of MAPKs in LPS-induced RAW264.7 cells. Cells were pretreated with n-hexane extract (200 µg/mL) for 2 h following treatment of LPS (1 µg/mL) for the indicated times (1, 2, 6 h). Cell lysates were collected and protein expression and phosphorylation of ERK, P38, and JNK were analyzed using western blotting. (a). The representative results of western blotting bands. (b-d). The relative phosphorylation levels of ERK (b), P38 (c), and JNK (d) were quantified using image J and normalized to that of the unphosphorylated form. The data are presented as means ± SD of three independent experiments. *p<0.05, ****p<0.0001 : Effect of n-Hexane Extract of Camellia Oleifera Fruit Hull on Inflammatory Responses in LPS-Induced RAW264.7 Cells : Science and Education Publishing

Figure 5. Effects of n-hexane extract of C. oleifera fruit hull on the phosphorylation level of MAPKs in LPS-induced RAW264.7 cells. Cells were pretreated with n-hexane extract (200 µg/mL) for 2 h following treatment of LPS (1 µg/mL) for the indicated times (1, 2, 6 h). Cell lysates were collected and protein expression and phosphorylation of ERK, P38, and JNK were analyzed using western blotting. (a). The representative results of western blotting bands. (b-d). The relative phosphorylation levels of ERK (b), P38 (c), and JNK (d) were quantified using image J and normalized to that of the unphosphorylated form. The data are presented as means ± SD of three independent experiments. *p<0.05, ****p<0.0001

Journal of Food and Nutrition Research. 2023, 11(4), 301-309 doi:10.12691/jfnr-11-4-4