Figure 3. Effects of n-hexane extract of C. oleifera fruit hull on the expression of inflammatory proteins in LPS-induced RAW264.7 cells. Cells were pretreated with the indicated concentrations of n-hexane extract (0, 25, 50, 100, and 200 µg/mL) for 2 h following treatment of LPS (1 µg/mL) for 24 h. Cell lysates were obtained and the protein expressions of iNOS, COX2, and GAPDH were analyzed by western blotting. (a). The representative results of western blotting bands. (b-c). The relative protein expressions of iNOS (b) and COX2 (c) were quantified using image J and normalized to GAPDH. The data are presented as means ± SD of three independent experiments. ****p<0.0001 compared with control group, ##p<0.01, ###p<0.001, ####p<0.0001 compared with LPS group

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Effect of n-Hexane Extract of Camellia Oleifera Fruit Hull on Inflammatory Responses in LPS-Induced RAW264.7 Cells

Chuanqi Xie, Shufen Wang, Xinying Lin, Fan Yang, Juwu Hu, Jing Wu, Wei Xiong, Lei Wu

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