Abstract

Sustainability of winter wheat yield under soluble sugar content conditions depends on improvements in crop photosynthetic characteristics and, crop yield. Experiment comprised of seven nitrogen treatments: 0 kg ha⁻¹, 90 kg ha⁻¹, 180 kg ha⁻¹, 210 kg ha^-1, 240 kg ha⁻¹, 270 kg ha⁻¹, and 300 kg ha⁻¹. The grain yield was increase by 25%, respectively. Our results indicated that implication of a proper increased nitrogen application rate, soluble sugar content, sucrose content and starch content were changed greatly, but when the threshold value was reached, the growth rate was decreased. Among them, the accumulation of soluble sugar contnt in the middle and late stages were increased, and the sucrose content and starch content were increased in each period after anthesis. The contents of each protein component were increased, the albumin was highest in 240 kg ha^-1, and the globulin was highest in 270 kg ha^-1 compaired to other nitrogen rate, respectively. The prolamin and glutenin (storage protein) were the highest in 300 kg ha^-1 and also in in 240 kg ha^-1. The protein content was improved in 300 kg ha^-1, 240 kg ha^-1, and protein yield 240 kg ha^-1 was significantly improved compared to other nitrogen application rates. The dynamic changes showed that the application of nitrogen fertilizer increased the protein contents in each period after flowering stage, but the effect was increase to 240 kg ha^-1 in began was weaken and the albumin were regulated by nitrogen fertilizer in the early stage.

1. Introduction

Winter wheat (Triticum aestivum L.) is one of the most important food crops in the world. It is an important crop in the southeast Loess Plateau of China, accounting to a report about one-fifth of food production. Nevertheless, the yields in this areaa are often unstable owing to limited and uneven precipitation distribution in the fallow and growing season ¹. In winter wheat production the major task is to use method which could effectively enhance the soil moisture consumption. Drill sowing method has been used for dryland wheat production. Compared to wide-spase sowing, and drill sowing, can increase the growth of wheat at various growth stages, and improve the efficiency of water uptake and increase yield ². Nutrients management, and cultural practices greatly influence Photosynthesis in crops. Wise space sown is an adapted form of drill sowing in which the seeds are evenly distributed, and in the same level ³. Appropriate water management can improve the light transmittance and photosynthetic area of wheat in the later growth periods, promote the accumulation and translocation dry matter, in wheat before heading ⁴. Therefore, it is of great importance to investigate the high-yield cultivation of wheat while regulating the water content at the tillering stage. Photosynthesis is necessary to accumulate energy and dry matter for plant growth and development. Photosynthesis of wheat leaves accounts for 95% of yield. The influence of environmental factors on photosynthesis can be reflected by chlorophyll light ⁵. Photosynthesis, transpiration, stomatal opening, and other physiological processes interact with each other ⁶. Previously, researchers believed that flag leaf photosynthesis provided most of the assimilates for grain filling ⁷, However, some studies have shown that wheat ear is an important source of photosynthetic carbon assimilation during grain filling, especially in the case of water deficit ^{8, 9}. It is now generally accepted that ear photosynthesis in wheat is the main contributor to the final grain yield ¹⁰. Compared with flag leaves, the photosynthetic rate of wheat spike decreased less under water deficit condition ^{10, 11}. Several traits may support better photosynthetic performance of wheat ear than flag leaf under drought conditions.First, wheat is closer to the grain, which is the main photosynthetic sink compared to flag leaves ⁵. The sowing methods used in wheat cultivation include DS, WSS, which affect the yield of wheat ⁶. Drill sowing in dry land could increased stem height, lengthen the growth time of functional green leaves, improve spike quality and yield compared with that in flat land ¹². The wide space sowing is a three-dimensional uniform sowing in which seeds are evenly distributed despite sowing in rows ³. Tiller number and percentage of productive tillers, leaf area index, dry weight, and yield were increased by uniform sowing without a significant decline in grain protein. In China excessive applicaion of N fertilizers by farmers is widely practiced to sustain further increase grain yield, but grain yield does not keep synchronous increase with excessive N application ¹³. Nitrogen application during the wheat growing season generally exceeds 340 to 300 kg Nitrogen ha⁻¹ however, some farmers use rates as high as 750 kg N ha^{−1 14, 15, 16}.

In this study the objects were as given; (i) To find the photosynthetic traits yield, (ii) The improvement of sugar and protein content, and the quality of wheat was improved and Wide space sowing (WSS) production is mainly through the increase in the number of spikes to increase yield. (iii) To optimize N rates under the sowing methods which produced the highest yield of wheat crop.

2. Materials and Methods

The field experiments were conducted during the winter season (2019-2020) at the experimental station of Shanxi Agricultural University. The experimental area is geographically located in Wenxi county (34° 35 'N and 110°15' E.), Shanxi Province, China and characterised by, hilly, semi arid area. In these regions, precipitation is the sole source of moisture and the mean annual rainfall ranges from 450-630 mm, of which 60-70% precipitation is concentrated in July-September. Winter wheat is planted in early to mid- October and harvested in early June, while corn is planted in mid to late June and harvested in early October of the same year.

The experiment had a single-factor randomized block design. The experiment comprised of three sowing methods: Wide-space sowing (WSS), (row and sowing spacing were 25 and 8, 2BMF-12/6, tillage, auto-fertilization), DS (row and sowing spacing were 20 and 3 cm, 2BXF-12Seed driller). The area of each plot was 2.5 m × 40 m=100 m2 and repeated each treatment three times, Winter wheat (Triticum aestivum L.), cultivar ‘Liangxing-99’ were obtained from the Wenxi Agriculture Bureau, Wenxi, China. The planting density was 225 × 104 plant ha⁻¹. seven Nitrogen application rates (0 kg ha⁻¹, 90 kg ha⁻¹, 180 kg ha⁻¹, 210 kg ha⁻¹, 240 kg ha⁻¹, 270 kg ha⁻¹, and 300 kg ha⁻¹). Pure P₂O₅ and K₂O were applied at the rate of 150 and 75 kg ha⁻¹ respectively. The seeds were sown on September 25, 2019, and September 25, 2020. Approximately 20-30 cm of the post-harvest stubble was left intact to increase soil organic carbon and reduce evaporation, and the remaining wheat stubble (20-30 cm) was plowed to a 25-30 cm depth using a rotator at the beginning to middle of July by deep plowing, as described by ¹⁷.

The water meter was used to record the water amount accurately each time, and the water amount was 60 mm (1.92 m³/ plot). At the end of each the growth season of wheat is 1-2 times from green to jointing, and 2-3 times from flowering to grain filling. Prevention and control of diseases and insect pests, other management with conventional high-yield fields.

The various photosynthesis attributes including net Photosynthesis Rate (P_N), transpiration rate (E), carbon dioxide concentration (Ci), and stomatal conductance (C_i), (g_S) of upper most fully opened leaf was measured using a hand-held portable photosynthesis machine (CI-340 system USA). These measurements for wheat functional leaves were carried out in the morning from 9:00 - 11:00 a.m on a bright sunny day using in each growth period. Three samples were continuously measured for each treatment, and the test data were the average value measured throughout the day. In each treatment plants (20) dry biomass was recorded. At physiologicalmaturity, the ears were cut, save in the mesh bag, and were dried to investigate the number of spikes per unit area, average grain number per spike, and 1000-grain weight were recorded. Another 20 m² area was harvested to determine the grain yield (kg ha^-1).

After flowering period listed growth consistent and the same day flowering of wheat spike and peeling grain were placed in the oven dried at 105 °C for 20 mins and then at 80 °C for 12 hours for dry weight. The quality and speed of weighing samples greatly affect the overall quality of the test. Then the grain was weighed and phenol method was used to determine the content of sucrose, and ketone color method was used to determine the total soluble total sugar content. H₂SO₄-H₂O₂-Phenol blue color method was used to determine the seed protein.

Grain to leaf ratio was calculated according to ¹⁸, using Equations 1 and 2:

(1)

(2)

At maturity, ten plants from each plot were randomly sampled from the inner rows for the determination of yield components such as ear number, seed number per ear and weight of thousand seed. Plot grain yield was determined by harvesting all plants in the area of 20 m², shelled using machine, and the grain was air-dried for the determination of grain yield.

The continuous extraction method was used to determine the protein components of grains, and the nitrogen content ×5.7 was the protein content. Repeat 3 times for each sample. The samples were dried at 105°C for 30 min and then weighed at 75°C. The seeds at maturity were naturally air-dried without drying. After grinding, nitrogen content of plant samples was determined by H₂SO₄-H₂O₂-indiophenol blue colorimetric method.

Albumin

The 0.255g crushed sample was placed in a centrifuge tube, and added 2 mL water. After shaking, centrifugation was performed at 3000 r for 5 min. The supernatant was poured into the digestive tube, and repeated 3 times. Determination of nitrogen content by H₂SO₄-H₂O₂-indiophenol blue colorimetric method.

Globulin

Add 2 mL 10%NaCl to the residue in the centrifuge tube after extraction of aqueous solution, centrifuge at 3000 R for 5 min after shaking, and pour the supernatant into the digestive tube, repeat 3 times. Determination of nitrogen content by H₂SO₄-H₂O₂-indiophenol blue colorimetric method.

Gliadin

Add 2 mL 70% alcohol (calculated by weight ratio) to the residue after extraction of salt solution, then shake for 2 min, put the mixture in hot water at 80℃ for 30 min, take out the centrifuge tube, centrifuge at 3000 r after shaking for 5 min, Then, the supernatant was poured into the digestive tube, repeat 3 times. Determination of nitrogen content by H₂SO₄-H₂O₂-indiophenol blue colorimetric method.

Glutenin

Add 2 mL 0.2% NaOH was added to the residue after alcohol extraction, followed by centrifugation at 3000 R for 5 min after shaking, and the supernatant was poured into the digestive tube, repeated 3 times. Determination of nitrogen content by H₂SO₄-H₂O₂-indiophenol blue colorimetric method.

The data were subjected to analysis of variance (ANOVA) using DPS and SAS 7.5 and the significant difference between treatment means were compared using least significant difference (LSD) test at P = 0.05. All graphs were drawn using Microsoft Excel 2010.

3. Results

The soluble sugar content, sucrose content, and starch content in grain of wide-space sowing wheat increased gradually with the increase of nitrogen application rate, and N300 was the highest. The soluble sugar content of N300 and N270 was significantly different from that of other nitrogen application rate treatments, and the sucrose content of N180, N210, N240, N270, and N300 was significantly different from that of N90 and N0. Starch contents of N240, N270 and N300 were significantly different from those of N210, N180, N90 and N0. It can be seen (Table 2).

Content of soluble sugar, sucrose and starch was significantly regulated by nitrogen application rate, but when the nitrogen application rate reached a certain critical value, the content increased slightly.

The of CO₂ concentration of the flag leaves in different treatments were showed a gradually declining trend with the grouting process and the 0-7D, with increase in the amount of N-nitrogen fertilizer, the net photosynthetic rate (P_N) increased showing a single peak curve at the 7-14 days after anthesis (Figure 3_A).

Days after anthesis the P_N value was still higher at N300 but was not significantly different from 270 kg ha^-1 and 240 kg ha^-1. Days after anthesis 21_d and 28_d P_N was increased with N application and the flower 21_d was higher with 240 kg ha^-1 as compared to other treatments. After 28_d after anthesis, the Pn was still the highest with N240, but the difference was not significant compared to 180 kg ha^-1, 210 kg ha^-1, 270 kg ha^-1, and 300 kg ha^-1. Overall, the application of N fertilizer enhanced the Pn days after anthesis, but the effect of high nitrogen treatments 270 kg ha^-1, 300 kg ha^-1 was weakened in the later grouting and the wide-space sowing coupled with 240 kg ha^-1 sustained higher values at the whole grouting period. The intercellular CO₂ (C_i) of the flag leaves of DS and wide-spce sowing, was gradually decreased with the growing process, however, with the increase in the amount of N application, P_n showed a first decreasing and then increaseing trend days after anthesis. From the (Figure 3_B), it was obvious trhat the 240 kg ha^-1 treatment was significantly lower than other treatments on 7-14_d and 21_d after anthesis and 0 kg ha^-1 was the highest 240 kg ha^-1 and was lowest on 28 days after anthesis, but the difference was not significant compared to 270 kg ha^-1. The increase of nitrogen fertilizer can significantly reduce the intercellular CO₂ concentration in the leaves of the flags days after anthesis and the whole grouting period can be continued.

The g_S of the flag leaves in wide-space sowing, and drill sowing treatment was gradually decreased with advancement of growth stages while first increased and then decreased with increasing nitrogen application rate. From the (Figure 3_C),

It was evident that the 240 kg ha^-1 treatment resulted in higher g_s values than other treatments on the 14_d days after anthesis and, was the lowest in 0 kg ha^-1, and was highest in 240 kg ha^-1 at 21-28_d, but the difference was not significant. That increased N fertilizer can significantly increase the g_S of flower flag leaves but the effect lasts until the middle stage of growth and the effect was weakened in the later stage. Moreover, the E of flag leaves gradually decreased with the grouting process and the Tr of flag leaves was first increased and then decreased with the increase in N application days after anthesis. In the (Figure 3_D) indicated that after flowering the Tr values in 240 kg ha⁻¹ and 270 kg ha⁻¹ treatments were significantly higher as compared to the other treatments from 7-14 days and 21d, and 0kg ha⁻¹ was the lowest. After 28d, the values was greater in 240 kg ha⁻¹ than other treatments and 0kg ha⁻¹ was the lowest. These results indicated thatthe increase in N fertilizer can significantly enhance the Tr of flag leaves days after anthesis, and last for the whole growth period and the best effect was observed in 240 kg ha⁻¹ coupled with large wide-space sowingand drill sowing.

The plant height of wheat in wide-spce sowing treatment showed a steady increase and then decline with the increase in N rates (Figure 3_E). At jointing stage, plant height of N₂₄₀ treatment was significantly higher than other treatments and there was no significant difference between N₂₁₀ and N₂₇₀, N₁₈₀ and N₃₀₀, N₀ and N₉₀. After entering the jointing stage, the treatment with N₂₁₀ and N₂₄₀ showed the highest plant heigh, and the values in N₉₀ and N₀ was the lowest.

The N₂₄₀ increased the plant height and promoted the growth of wheat in the middle stage of wheat growth. Similar to plant height, he leaf area index LAI of wide-space sowing showed an increasind and then a declining trendat jointing and booting stages with an increase in nitrogen rates (Figure 3_F). Jointing, booting, 240 kg ha⁻¹ was significant It was significantly higher than other treatments, and N0 was the lowest. wide-spce sowing was beneficial to wheat growth and wide-spce sowing combined to N₂₄₀ had the largest leaf area index and the best growth condition in each growth stage.

The soluble sugar in wide space sowing decreased gradually with grain filling, but increased continuously with the increased of N rate at each stages after anthesis (Figure 4_A). From 5_d to 30_d after anthesis, N₃₀₀ treatment had the highest soluble sugar content, but other treatments (except N₀) had no significant difference, and N₀ treatment had the lowest soluble sugar content. At 35_d after anthesis, there was no significant founded in N₀.

In conclusion, increasing nitrogen fertilizer was beneficial in enhancing levels of soluble sugar at the middle and late stage, which could last until 25_d after anthesis, and provided conditions for starch synthesis. However, there was no difference between N₂₄₀, N₂₇₀ and N₃₀₀, and the effect of nitrogen fertilizer was weakened in the late stage. During grain filling, the sucrose content of wide space sowing wheat decreased gradually, but showed an increasing trend with the increase of nitrogen application rate, and maintained the highest level on the 5th day after anthesis (Figure 4_B). The content of sucrose in different periods after anthesis could be increased by increasing nitrogen application rate. On the 5th day after anthesis, sucrose contents of N₃₀₀ was higher as compared to restof the treatments. On the 15 day after anthesis, the highest sucrose content was found in N₂₇₀, followed by N₂₁₀, N₂₄₀, N₃₀₀. At 25_d after anthesis, N₃₀₀ had the highest value, but it was statistically similar to that in N₂₄₀, N₂₇₀ treatments. At 35_d after anastomosis, N₃₀₀ treatment still had the highest value, but there was no significant difference among different treatments. In conclusion, increasing nitrogen fertilizer can promote sucrose synthesis, and lay the foundation for starch accumulation, especially in the early stage of grain filling, and in the middle stage of high nitrogen treatment N₂₄₀, N₂₇₀, and N₃₀₀, there is no significant difference, and the effect of nitrogen fertilizer is weakened in the late stage. The soluble sugar of wide sowing wheat increased gradually with grain filling, and the starch content at different stages increased continuously with increase in N rate (Figure 4_C). The starch of N₃₀₀ treatment was highest, and the starch content of N₀ treatment was the lowest. Compared to other nitrogen treatments, starch content of N₃₀₀, N₂₇₀ and N₂₄₀ were increased significantly from 15_d to 35_d after anthesis. Therefore, increasing nitrogen fertilizer can promote starch accumulation and increase grain weight, and the effect was significant in the middle and late stage, but there was no significant difference among high nitrogen treatments N₂₄₀, N₂₇₀ and N₃₀₀, respectively.

The protein content of wide space swing wheat showed a trend of decreasing first and then increasing with the filling process and the protein content was the lowest 15 day after anthesis (Figure 5_A). The protein content at different stages after flowering N rate increased. The grain protein content of increased nitrogen fertilizer had a greater impact, but there was no significant difference in the regulatory effect between high nitrogen treatment after 5_d, 15_d, 25_d, 35_d, and N₂₄₀, respectively. Therefore, choosing appropriate nitrogen application amount was more beneficial to efficient production. The content of albumin was the highest at 5day after anthesis and decreased with the development and maturity of grains. Compared to other components, the globulin content was the lowest and began to 15day after anthesis. The content of gliadin and glutenin were increased continuously with the highest value of N₃₀₀ and the lowest value of Nitrogen at different time after flowering.

The albumin was significant than other treatments on 5day after anthesis N₃₀₀, 15day after anthesis N₃₀₀ and N₂₇₀, and 25_d-35day after anthesis N₃₀₀, N₂₇₀ and N₂₄₀ (Figure 5_B). The globulin protein content was significantly higher than that of N₃₀₀, N₂₇₀, N₂₄₀, N₂₁₀, N₁₈₀ treatment on 5 day after anthesis than that of N₉₀, and N₀ treatment and was significant higher than that of other treatments on 15_d-35 day after anthesis (Figure 5_C). The content of gliadin increased with the increase of N rate. At 5 day after anthesis, difference between nitrogen application amounts was not significant, and at 15_d-35 day after anthesis, (Figure 5_D). Glutenin was significant N₃₀₀, N₂₇₀, N₂₄₀, N₂₁₀ and N₁₈₀ treatments than in N₉₀ and N₀ treatments on 5day after anthesis but was significantly higher in N₃₀₀ and N₂₇₀ treatments than in other treatments on 15day after anthesis, in N₃₀₀ and N₂₇₀ treatments than in other treatments on 25day after anthesis, and in N₃₀₀, N₂₇₀ and N₂₄₀ than in other treatments on 35 day after anthesis (Figure 5_E). The adding N rate can improve increased of grain protein. Albumin was regulated N fertilizer in the early stage of filling, globulin was more regulated throughout the filling stage, while gliadin and glutenin are more sensitive to N rate in the late stage of filling.

The N fertilizers rates were significantly effected the final grain yield and compositional factors of yield evident by an increased ear number, yield and increased ear number (Table 2). The spikes number, ear shots, and the thousands grains weight, and grain yield showed an afirst increase and then decreasing trend with application of nitrogen fertilizer amount. The yield and yield components were significantly higher in N₂₄₀ followed by N₂₁₀ under dirll sowing when compared to the other nitrogen treatment. Wide-spae sowing, and drill sowing methods optimized the output of the three elements at the same time of wide-space sowing coupled to 240 kg ha⁻¹ and dirll sowing casting 210 kg ha⁻¹ to achieve the increase of the output. Both dirll sowing and wide-space sowing significantly increased spike number and yield. With the increased of nitrogen application rate, the three factors of yield increased first and then decreased. The yield and three elements of wide-spase sowing were the highest at 240 kg ha⁻¹ and 210 kg ha⁻¹, respectively.

The effects of nitrogen fertilizer on the contents of protein and its components in grains were significantly different (Table 3). The contents of albumin and globulin (soluble protein) increased firstly and then decreased with the increase of nitrogen application rate. The highest albumin was N₂₄₀, and the difference between N₂₇₀ and N₃₀₀ was not significant. The highest globulin was noted in N₂₇₀, and the difference between N₁₈₀, N₂₁₀, N₂₄₀ and N₃₀₀ was not significant. The contents of gliadin and glutenin (storage protein) increased with the increase of nitrogen application, and N₃₀₀ was the highest, but the difference of gliadin content was not significant compared with N₂₇₀, and the content of glutenin N₃₀₀ was significantly higher than that of other nitrogen treatments. The grain alcohol ratio of N₂₄₀ was the highest, but the difference was not significant compared with that of N₉₀ and N₁₈₀. The protein content of N₃₀₀ and N₂₄₀ was significantly higher than that of other nitrogen treatments. Protein yield of N₂₄₀ was significantly higher than that of other n treatments. The results showed that nitrogen fertilizer regulated storage protein significantly, which was more beneficial to improve the quality of storage protein.

4. Discussion

The wide space sowing is a newly introduced sowing method. Furrow sowing is gaining popularity for wheat because seeds are sown in furrows that facilitate fertilization and effectively improve nutrient utilization ¹⁴. Different sowing methods affects canopy structure by altering the arrangement and spacings of plants. The better plant distribution effectively intercepts radiation and improves photosynthesis efficiency and growth ³. The results of our present study showed that the wide space sowing and furrow sowing can improve radiation interception as shown by the higher leaf area index under these sowing methods. Photosynthetic active radiation was found positively related to the leaf area index and the number of spikes ¹³. The results from this study showed that the grain yield and other yield traits under WSS, DS, and stereoscopic sowing were higher than the drill sowing. The highest yield was attained by WSS, followed by FS. The that sowing methods mainly effected yield by influencing the number of spikes ¹⁹. The furrow sowing was proved beneficial in maintaining a higher photosynthesis rate. In a previous study indicated that furrow planting increased chlorophyll content and photosynthesis as a result of which dry matter accumulation increased in each growth period ^{12, 20}. The furrow sowing produces stronger seedlings before winter, increases tiller numbers from booting to maturity stage, increases earning rate by 3.5%, and significantly increases yield ^{21, 22}. In a study it was believed that C_i and P_N values could be used as indicators to determine whether photosynthesis is limited by stomatal closure or metabolic damage. Our results showed that the decrease in stomatal conductance caused by the partial closure of stomata in the DS treatments was the main reason for the decrease in photosynthetic rate, which was mainly manifested by the decrease of P_n and the increase of C_i. The gs value and Pn value of each treatment had good synchronization, which showed that they decreased with the decrease in water content, and both the Pn and gs values recovered after rewatering, with the WSS and DS treatments having a high recovery level ²³. Stomatal conductance is an important biological process reflecting carbon accumulation and transpiration in plants, and CO₂ flows into photosynthetic sites through stomata ²⁴. Inconsistent results were found in the analysis of stomatal distribution in ear buds of spring wheat. It should be noted that under moderate water deficit conditions, stomata were distributed in the front and back of the wheat ear buds, and in the lower part of the front of the wheat ear buds. The characteristics of the paraxial stomata may contribute to the absorption of CO₂ released by grain respiration, and the recycling of respiratory CO₂ is considered to be a key process for complete assimilation in wheat ears ^{25, 26}. Previously, it was reported that that the wide-precision planting significantly increased the leaf area index and interception of photosynthetic active radiation of winter wheat ³ ,it was reported that the uniform sowing was more conducive for light interception for the production of more grain. The plant height was highest indirect sowing whereas the leaf area index was lowest, which might indicate the effect of shading under direct sowing methods as compared to uniform sowing, furrow sowing, and wide space sowing. The photosynthetic rate Tr, and gs of the flag leaves were higher under WSS, FS sowing especially at the end of flowering ²⁷. Lower reduction in both flag leaves and spikes under water deficit compared to during the middle and late grain-filling stages. In contrast, gs in flag leaves were more sensitive to drought than that of spikes ²⁸. The decline in P_N may occur due to decreased T_R in wheat, and a similar result was observed in mulberry ²⁹. However, variations of gs would produce differences in E for wheat leaves ³⁰. Our study indicated that under the WSS, and DS, wheat crop produced a more favorable canopy structure with higher photosynthesis and grain yield. Higher yield depends on the higher photosynthesis rate and thus increasing photosynthetic rate is the main objective to increase production. Furthermore, the photosynthesis rate is positively related to leaf mass per unit area. However, the excessive growth at the early stages is not much favorable under dryland conditions. It is also manifested by the highest plat height while low leaf area index by drill sowing as compared to other sowing method ³¹.

Th results indicated that the optimum nitrogen application rates vary with the sowing method in terms of increasing production. Compared with other treatments, the N amount of 210 kg ha^-1 and 240 kg ha^-1 combined with wide-space sowing and furrow sowing resulted in higher tiller numbers, optimized the yield components and improve the grain yield. This might be because row spacings are larger in the wide-space sowing than the furrows, whereas, space for the growth of single plant reduced and competition between plants for natural resources has been increased. Therefore, the effect of higher nitrogen application rate proved better because fertility has improved plant growth by promoting the absorption and transportation of water and fertilizer by the root system ³². In addition it also facilitated the deep fertilizers application, improved radiation interception and water utilization ^{21, 33}. Leaf area is an important canopy structural feature closely associated with the ability of leaves to collect light and photosynthesize ³⁴. As compared with drill sowing, the leaf area index of winter wheat increased under wide space sowing, especially in the middle and late growth period. The differences in leaf area under the different planting methods could be attributed to the differences in the canopy distribution. Canopy with wider spacing intercepts light more properly with a higher photosynthesis rate and leaf area index ²⁷. In this study, wide space sowing proved better than drill sowing in photosynthesis. This may be due to the reason that wide seed spacings not only expand the growth space of a single plant and reduce the competition of plants for natural resources ¹⁹. Our study indicated that under WSS and, DS the wheat crop produced a more favorable canopy structure with higher photosynthesis and grain yield. Higher yield depends on the higher photosynthesis rate and thus increasing photosynthetic rate is the main objective to increase production. Furthermore, the photosynthesis rate is positively related to radiation use efficiency and leaf mass per unit area. However, the excessive growth at the early stages is not much favorable under dryland conditions. It is also manifested by the highest plat height while low leaf area index by drill sowing as compared to other sowing methods.

5. Conclusion

To explore the optimum amount of nitrogen application in different nitrogen rates of winter wheat, improving flag leaf photosynthesis capacity, obtaining higher yield and grain protein content. The post-anthesis flag leaf photosynthetic rate, the transpiration rate, the intercellular carbon dioxide concentration, and flag leaf stomatal conductance were significantly increased. The wide-space sowing with nitrogen level N240 improved the photosynthetic characteristics of flag leaves, significantly increased the net photosynthetic rate, stomatal conductance, and transpiration rate of flag leaves after flowering, and significantly reduced the intercellular carbon dioxide concentration. The soluble sugar content, sucrose content and starch content changed greatly, but when the threshold value was reached, the growth rate decreased. Among them, the accumulation of soluble sugar in the middle and late stages increased, and the sucrose content and starch content increased in each period after anthesis. The content of each protein component increased, the albumin was highest in 240 kg ha⁻¹, and the globulin was highest in 270 kg ha⁻¹. The increased 1000-grain weight of each time after flowering, significantly increased panicle number and yield. The nitrogen transport before flowering and the nitrogen accumulation after flowering. The nitrogen metabolism of the plants was improved, which was beneficial to the improvement of sugar and protein content, and the quality of wheat was improved.

Acknowledgements

The authors are thankful to ‘Modern Agriculture Industry Technology System Construction’ (No. CARS-3124). The National Key Research and Development Program of China (No. 2018YFD020040105), The Sanjin Scholar Support Special Funds Projects, National Natural Science Foundation of China (No. 31771727), The ‘1331’ Engineering Key Innovation Cultivation Team- Organic Dry Cultivation and Cultivation Physiology Innovation Team (No. SXYBKY201733) for financial support of this study.

References