Figure 1. ARC inhibits tube formation and induces reduction of angiogenesis-related adhesion molecules in tube-forming HUVECs. A:HUVECs were sandwiched between two layers of collagen gel and induced to form blood vessel-like tubes. The cells were treated with various concentrations of ARC (3.13, 12.5, and 50 µg/mL). Tube-forming HUVECs in a 2-D culture model were observed at 6, 12, and 24 h. Each experiment was repeated at least three times and representative data are shown. Bar indicates 100 μm. B: ARC blocked the tube formation of HUVECs in a 2-D culture model. The tube-forming cells were treated with various concentrations of ARC for 24 h and then fixed. The fixed cells were stained with 0.1% toluidine blue. (a) 0 µg/mL, (b) 3.13 µg/mL, (c) 12.5 µg/mL, (d) 50 µg/mL. Representative photos are shown. Bar indicates 200 μm. Values are expressed as means ± SE (n = 3). *P < 0.05 vs. the control group.C: Cellular proteins were collected from tube-forming HUVECs that were treated with various concentrations of ARC(3.13, 12.5, and 50 µg/mL) for 12 and 36 h. Changes in VE-cadherin and PECAM-1 were analyzed by Western blotting. Each experiment was repeated at least three times and representative data are shown

From

Artepillin C Suppresses Angiogenesis by Inhibiting Tube-Formation and Inducing Apoptosis of Endothelial Cells

Mok-Ryeon Ahn, Sun Wook Kim, Shigenori Kumazawa, Toshiro Ohta

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