Figures index

From

Purification of an Antiproliferative Lectin from Erophaca Baetica (Leguminosae) Seeds

Cristina Megías, Isabel Cortés-Giraldo, Julio Girón-Calle, Manuel Alaiz, Javier Vioque

Journal of Food and Nutrition Research. 2013, 1(5), 87-91 doi:10.12691/jfnr-1-5-2
  • Figure 1. Gel filtration chromatography of E. baetica albumin extract using Superose 12 (A). Fractions corresponding to the first peak were pooled together and re-chromatographed twice more (B and C)
  • Figure 2. SDS-PAGE of the putative E. baetica lectin purified by gel filtration chromatography as shown in . Molecular weight standards are shown on the left
  • Figure 3. Effect of E. baetica lectin on the proliferation of human leukemic THP-1 cells. Cells were incubated in the presence of 3, 6, 9, 12 μg / mL lectin for up to 5 days. Positive control corresponds to cells treated with vehicle (PBS), negative control corresponds to cells treated with the apoptosis inducing agent camptothecin (20 M). Results are the mean ± SEM for n = 6. *** (p < 0.005), significantly different as compared to positive control
  • Figure 4. Dendrogram produced by cluster analysis of the amino acid composition of different legume lectins. A. mongholicus (Yan et al., 2005), C. arborescens (Bloch et al., 1976), O. arenaria (AN.: AAL79163.1), M. sativa (AN.: CAA76366.1), L. sativus (AN.: CAD27485.1), V. faba (AN.: CAD27484.1), P. sativum (AN.: AAA33675.1), L. culinaris (AN.: CAC42124.2)