Figures index

From

Anticancer Effect of Medicinal Mushroom with Prooxidant Activity on Human Bladder Cancer Cells

Cristina Fox, Roger Yau, Muhammad Choudhury, John Phillips, Sensuke Konno

Journal of Cancer Research and Treatment. 2018, 6(2), 54-59 doi:10.12691/jcrt-6-2-5
  • Figure 1. Dose-dependent effect of PE on cell viability. 5637 cells were cultured with varying concentrations of PE (0-200 µg/ml) and cell viability was assessed in 72 h by MTT assay. Cell viability was expressed by the percent (%) of viable cells relative to controls (100%). The data are mean ± SD (standard deviation) from three separate experiments (*p <0.05 compared with control)
  • Figure 2. Assessment of severity of OXS. Cells were exposed to PE (100 µg/ml) or H2O2 (50 µM) for 3 or 6 h, and the amount of MDA formed (μM)) was assessed by LPO assay. All data are mean ± SD from three independent experiments (*p <0.05 compared with controls)
  • Figure 3. Inhibition of glycolysis by PE. Cells were treated with PE (100 μg/ml) for 72 h and subjected to HK and ATP assays separately. HK activity and ATP level are expressed by the % relative to respective controls (100%). The data are mean from three separate experiments (*p <0.05 compared with control)
  • Figure 4. Modulations of metabolic regulators by PE. Cells treated with PE (100 μg/ml) for 72 h were analyzed for three key metabolic regulators using Western blots. Autoradiographs of p-AMPK, p-Akt, and p-mTOR expressed in control and PE-treated cells are shown for comparison. Beta-actin is also shown as a loading control
  • Figure 5. Induction of apoptosis. Autoradiographs of two apoptotic regulators, bcl-2 and Bax, expressed in control and PE-treated cells are shown following Western blot analysis