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From
Abrogation by
Ginkgo
Byloba
Leaf Extract on Hepatic and Renal Toxicity Induced by Methotrexate in Rats
Ehab Tousson, Zeinab Atteya, Afaf El-Atrash, Ola I. Jeweely
Journal of Cancer Research and Treatment
.
2014
, 2(3), 44-51 doi:10.12691/jcrt-2-3-1
Figures 1A-1F
.
Photomicrographs of rat Liver sections in the different experimental groups stained with Haematoxylin & Eosin.
A:
Rat Liver sections in control group revealed normal structure of hepatocytes.
B:
Rat Liver section in GLE group revealed normal structure of hepatocytes (hp).
C:
Liver sections of MTX group showed moderate to severe lose of liver architecture, disturbance of the hepatocytes, congestion (Black arrows) in central veins (CV), atrophied, and vacuolated hepatocytes (White arrows).
D&E:
Liver sections in co-treated and post treated rat with GLE groups revealed a moderate to mild degree of improvement in hepatocytes where a few vacuolated hepatocytes (White arrows) and mild congestion in central veins were observed.
F:
Liver section in self treated showed severe lose of liver architecture, marked disturbance of the hepatocytes and strong marked hepatocellular vacuolation (White arrows)
Full size figure and legend
Figures 2A-2F
.
Photomicrographs of rat kidney sections in the different groups under study stained with Haematoxylin & Eosin.
A&B:
Rat kidney sections in control and GLE groups respectively showed normal structure of renal cortex which comprised renal corpuscles (White arrows), proximal and distal convoluted tubules.
C:
Rat kidney sections in MTX group showed glomerular damage (White arrows), vacuolization in tubular cells focal necrosis and cell infiltration (Black arrows).
D:
Kidney section of co-treated group with GLE revealed a good degree of improvement glomerular damage with minimal vacuolization(Arrow heads) in tubular cells.
E:
Rat kidney section in post treated group with GLE showed moderate organized tubular and glomerular structures except mild inflammatory infiltration (Black arrows).
F:
Kidney section of MTX self treated showed severe lose of kidney architecture, marked disturbance in glomeruli with strong inflammatory infiltration (Black arrows)
Full size figure and legend
Figures 3(A-F)
.
Photomicrographs of rat Liver sections in the different experimental groups stained with CD68-ir.
A&B
: Negative CD68-ir reaction in control and GLE respectively.
C:
Severe positive reactions for Cd68-ir in MTX group.
D&E:
Mild to moderate positive reactions for CD68-ir in Co-treated and post treated rats with GLE respectively. F: Strong positive reaction for CD68-ir in MTX self treated rats
Full size figure and legend
Figures 4(A-F)
.
Photomicrographs of rat kidney sections in the different experimental groups stained with CD68-ir.
A&B
: Negative CD68-ir reaction in control and GLE respectively.
C:
Severe positive reactions for Cd68-ir in MTX group.
D&E:
Moderate to mild positive reactions for CD68-ir in Co-treated and post treated MTX with GLE respectively. F: Strong positive reaction for CD68-ir on the kidney in MTX self treated rats
Full size figure and legend