Figure 3. Chromatographic separation of crude extract. The solvent system of n-hexane and ethyl acetate (5:1) was chosen for chromatography. The separated bands on the TLC plate were visualized under UV (A) and FeCl₃ spray (B). A total of 322 fractions were eluted. Each fraction was subjected to TLC analysis to check the banding pattern of the compounds. Fractions having almost similar bands were combined. Ten combined fractions (F1 to F10) obtained were tested for their urease inhibitory effect. F3, F4, and F10 were found to be the same as negative control (Con⁻) in colorimetric analysis (C). D: The banding pattern of phytochemicals present in combined fractions on a TLC plate. Fractions (F3, F4) shared four common bands, two of them were more intensified (bracket) and differed in two separate bands (arrowheads). The common bands enclosed by the bracket might be the targets that are responsible for urease inhibition

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Non-flavonoid Polyphenol of Allium cepa Tuber Inhibits Bacterial Urease and the Proliferation of Lung Cancer Cells

Saadullah, Aziza Sultana, Md. Najem Uddin, Md. Atikur Rahman, Most. Reshma Akter, Ismot Ara Shathi, Mst. Bobita Yeasmin, Md. Mahadi Hasan Lavlu, Monika Rani Saha, Aktar Uzzaman Chouduri

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