Figures index

From

Possible Anticancer Effect of Bioactive Extract Isolated from Agrocybe Chaxingu on Human Bladder Cancer Cells

Jonathan Rockoff, Akhil Saji, Jonathan Kroop, Muhammad Choudhury, Sensuke Konno

Journal of Cancer Research and Treatment. 2023, 11(1), 1-5 doi:10.12691/jcrt-11-1-1
  • Figure 1. Effect of CHX on 5637 cell viability. Cells were treated with varying concentrations of CHX (0-500 μg/ml) for 72 h and cell viability was determined by MTT assay. Cell viability was expressed by the % of viable cell numbers in CHX-treated cells relative to that in control cells (100%). All data represent mean ± SD (standard deviation) from three independent experiments (*p <0.05 compared with control).
  • Figure 2. CHX-induced OXS. 5637 cells exposed to CHX (350 μg/ml) for 3 or 6 h were subjected to LPO assay. Severity of OXS is determined by the amount of MDA formed (μM), and the data are mean ± SD from three separate experiments (*p <0.05 compared with control).
  • Figure 3A. CHX-induced G1 cell cycle arrest. Cells treated with CHX (350 μg/ml) for 72 h were subjected to cell cycle analysis. The cell distribution (%) at each cell cycle phase in control and CHX-treated cells is shown. The data are mean ± SD from three separate experiments but only mean values (no error bars) are shown (*p < 0.05 compared with control).
  • Figure 3B. CHX-induced G1 cell cycle arrest. Expressions of two G1-specific cell cycle regulators, p21WAF1 and p27Kip1, were analyzed by Western blots. Autoradiographs of these regulators in control and CHX-treated cells are shown for comparison. Beta-actin is also shown as an internal loading control.
  • Figure 4. CHX-induced apoptosis. Cells treated with or without CHX (350 μg/ml) for 72 h were analyzed for two apoptotic regulators, bcl-2 and Bax, using Western blots. Autoradiographs of these regulators in control and CHX-treated cells are shown for comparison. Beta-actin is also shown as a loading control.