Avian infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that causes an economically important respiratory chicken disease. The disease mainly controlled by vaccination. However conventional vaccinations increased the spread of the virus by latency. Therefore the aim of this study was to design multi epitopes vaccine against glycoprotein D of ILTV using immunoinformatics tools. The envelope glycoprotein D sequences were retrieved from the National Center for Biotechnology Information (NCBI) and aligned using Bioedit software for conservancy. The prediction of B and T cell epitopes were performed using Immune Epitope Database (IEDB) analysis resources. Homology modeling and docking were also performed to predict the binding affinity of the predicted epitopes to the chicken alleles. B cell prediction methods proposed nineteen linear epitopes, among them twelve epitopes were on surface and eleven antigenic epitopes using Bepipred, Emini surface accessibility and kolaskar antigenicity methods, respectively. However, only seven epitopes fulfilled the B cell prediction methods. Among these seven epitopes, two epitopes namely 256PRPDSVPQEIPAVTKK271 and 226 RHADDVY 232 were proposed as the top B cell epitopes. For T cells, three epitopes namely 24STAAVTYDY32, 20FASQSTAAV28 and 353FAAFVACAV361 were proposed as cytotoxic T cells (CTL) epitopes due to their great allele’s linkage to MHC class I alleles. MHC class II alleles extensively interacted with multiple epitopes. The best predicted epitopes were 88FEASVVWFY96, 212FQGEHLYPI220, 353FAAFVACAV361 and 137VDYVPSTLV145. Moreover, molecular docking revealed high binding affinity between chicken MHCI BF alleles and MHC1 docked epitopes (20FASQSTAAV28, 24STAAVTYDY32 and 353FAAFVACAV361) that indicated by the lower global energy scores. The In-silico analysis of ILTV glycoprotein D in this study suggested eight epitopes that could be a better choice as worldwide multi epitopes vaccine. These epitopes may effectively elicit both humoral and cell-mediated immunity. Furthermore in vitro and in vivo studies are required to support the effectiveness of these epitopes as vaccine candidates.
Infectious laryngeotracheitis (ILT) is a contagious respiratory disease of chicken caused by an enveloped virus with a double stranded DNA genome. Infectious laryngeotracheitis virus (ILTV) belongs to the family Herpesviridae and subfamily alphaherpersvirinae 1, 2. The disease is characterized by depression, conjunctivitis, sneezing, nasal exudate and, in severe cases, gasping, dyspnea and death 3, 4. The severe epizootic form of the disease may cause morbidity up to 100% and 70% mortality 5. ILT virus possesses at least 10 envelope glycoprotein genes, including the UL27 and US6 genes, encoding glycoprotein B (gB) and glycoprotein D (gD), respectively that are highly conserved herpesvirus structural glycoproteins 6. Glycoprotein B is essential for infectivity and is involved in membrane fusion and virus penetration. Glycoprotein D is essential for most herpesviruses and functions as a receptor for virus binding to susceptible cells 6, 7. In addition, gB and gD elicited neutralizing antibodies and cell-mediated immune responses and has been shown to be a candidate antigen for recombinant vaccines 6, 7.
Vaccination is generally considered to be the most effective method of preventing infectious diseases 8. ILTV was the first poultry pathogen controlled by vaccination. However, it is still a major problem in areas in which dense bird populations exist 4. Chickens are vaccinated against ILTV using attenuated strains by multiple passages either in embryonated eggs (chicken embryo origin, CEO) or in tissue culture (tissue culture origin, TCO) 4. Although live attenuated ILTV vaccines have been used widely, the disease remains a major concern in the poultry industry because it often occurs due to the production of latent infected birds 4, 5. Peptide-based vaccines combines immunoinformatic prediction tools with laborious experimental computational validation, making it easier to identify epitopes in protein antigens that acted as a candidate vaccine 9. These vaccines are known to produce satisfactory results 8, 9, 10. Numerous computational studies addressed the safety, accuracy, feasibility and speed of these vaccines adequately 11, 12.
Recently multi epitopes vaccine using immunoinformatics tools was predicted for ILTV using glycoprotein B as an immunogenic target 13. However no studies were conducted in glycoprotein D for vaccine design against this virus. It is important to design a vaccine that works against all immunogenic proteins of ILTV using bioinformatics. In addition, this vaccine would be safe, effective and prevent birds from being carriers of the disease. Thus this study was a continuation of the prediction of an in silico vaccine from gD as a target protein against ILTV. In this study we aimed to predict effective multi epitopes vaccine against ILTV from glycoprotein D (gD) which is essential for virus binding and attachment with high safety and accuracy.
A total of four glycoprotein D (gD) sequences from virulent strains of ILTV were retrieved from the GeneBank of National Central Biotechnology Information (NCBI) (https://www.ncbi.nlm.nih.gov/protein/) database in September 2017 14. The accession numbers, date and region of collection of the retrieved strains were listed in Table 1.
Alignment of the retrieved sequences was performed using ClustalW in the BioEdit program version 7.2.5. to obtain conserved regions using multiple sequence alignment (MSA) 15. Moreover the retrieved strains were subjected to molecular evolution to demonstrate their common ancestors using MEGA7.0.26 (7170509) 16.
2.3. B-cell Epitope PredictionB cell epitopes were predicted from Immune Epitope Database analysis resource IEDB (https://www.iedb.org/) 17. BepiPred from immune epitope database was used to predict linear B-cell epitopes 18. The surface accessibility of the predicted epitopes was investigated using Emini surface accessibility prediction tools 19. While the antigenic epitopes were predicted through kolaskar and Tongaonkar antigenicity method 20.
2.4. MHC Class I Binding PredictionsThe reference sequence of glycoprotein D of ILTV was submitted to MHCI prediction tools in IEDB (https://tools.iedb.org/mhci/) to detect and analyze the binding of peptides to MHC class I molecules 17. To complete the analysis, the human alleles were used due to lack of chicken alleles in this tool. Artificial neural network (ANN) was used as prediction method 8, 21 and all peptide lengths were set as 9amino acids. The half maximal inhibitory concentration (IC50) values of the peptides binding to MHC-I molecule was calculated. Epitopes with IC50 binding affinity equal to or less than 300 nM were suggested as promising candidate epitopes.
2.5. MHC Class II Binding PredictionsAnalysis of epitopes that bound to MHC class II molecules was performed by the IEDB MHCII prediction tool at (https://tools.iedb.org/mhcii/) 17. For MHCII binding predication, human MHC class II alleles (HLA DR, HLADP and HLADQ) were used. MHC class II groove has the ability to bind to peptides with different lengths. In this study the NN-align method was used with IC50 equal to or less than 1000 nM to predict epitopes that interacted with MHC class II alleles 22.
2.6. Homology ModelingMUSTER server (https//zhanglab.ccmb.med.umich.edu/MUSTER/) was used for modeling the 3D structure of the glycoprotein D reference sequence (YP _182405.2) 23. The 3D modeled of BF alleles (BF2*2101, BF2*0401) was also created using Raptor X server (https://raptorx.uchicago.edu/) 24, 25, 26 after retrieval of protein sequences and PDB ID of chicken alleles (BF2 *2101 & BF2*0401) from the NCBI database (PDB: 4D0C, CAK54661.1 and PDB: 4D0C CAK54660.1). Chimera software 1.8 was used to display the 3D structures of the glycoprotein D reference sequence and BF alleles 27. The 3D structures of predicted peptides were designed using PEP FOLD3 (https://bioserv.rpbs.univ-paris-diderot.fr/services/PEP-FOLD3/) 28, 29, 30. While the 3D modeled epitopes was performed using PatchDock online autodock tools; an automatic server for molecular docking (https://bioinfo3d.cs.tau.ac.il/PatchDock/) by submitting PDB of ligands and receptors after homology modeling by Raptor X server and PEP FOLD3 31, 32. Firedock was used to select the best models 33. Visualization of the result was performed using the offline UCSF-Chimera visualization tool 1.8. 27.
2.7. Molecular DockingThe interaction between 3D model of BF alleles (BF2*2101, BF2*0401) and 3D model epitopes was performed by submitting them to PatchDock online autodock tools; an automatic server for molecular docking (https://bioinfo3d.cs.tau.ac.il/PatchDock/) 33. Firedock was used to select the top models 33. Visualization of the result was performed using UCSF-Chimera visualization tool 1.8. 27.
Clustal W was used to align the retrieved sequences to obtain conserved regions between the retrieved sequences. As shown in Figure (1-a) the alignment demonstrated conservancy between the sequences despite some changes in some amino acids sequences was reported. Phylogenetic tree was constructed using MEGA7.0.26 (7170509). The evolutionary divergence among each protein was analyzed. As shown in Figure 1-b. The Chines strains AGN482227 and AGN48385 clustered together and shared common ancestor. Moreover to two strains clustered with USA strain YP182405. However the Chinese strain AGN 48305 was far related to the other strains.
3.2. Prediction of B-cell EpitopesB-lymphocytes are differentiated into antibody-secreting plasma cell and memory cells. The B cells epitopes are characterized by being hydrophilic and accessible and flexible 34. The reference sequence of glycoprotein D was analyzed using Bepipred Linear Epitope Prediction, Emini surface accessibility and Kolaskar and Tongaonkar antigenicity method in the IEDB, based on the default threshold of the prediction method. As shown in Figure 2, Bepipred Linear Epitope Prediction method; the average score of mounting glycoprotein D to B cell was 0.306. In Emini surface accessibility prediction the average surface accessibility areas of the protein was scored as 1.000. The virtual threshold of antigenicity of the protein was 1.033. All values equal to or greater than the default thresholds of B cell prediction methods were suggested to be B cell binders (linear, surface or antigenic determinants). Thus based on the binding affinity to B lymphocytes, Bepipred Linear Epitope Prediction method proposed nineteen linear epitopes with different lengths. Among them twelve epitopes were predicted as surface epitopes using Emini accessibility and eleven epitopes were suggested to be antigenic. These epitopes and their scores in the B cell prediction methods were shown in Table 2. However, only seven epitopes successfully overlapped the B cell prediction methods. Two epitopes namely 256PRPDSVPQEIPAVTKK271 and 226 RHADDVY 232 were selected as the best B cell epitopes based on their conserved length, surface accessibility and antigenicity scores. The modeling the 3D structure of the glycoprotein D reference sequence (YP _182405.2) and the position and the 3D structure of these two epitopes in the reference sequence were shown in Figure 3 a-b.
Although MHC-I analysis tools supported epitopes prediction of many organisms, it lacked certain organisms such as chickens. However, several studies suggest some similarities between human HLA alleles and chicken MHC alleles 35. In the present study, the reference glycoprotein D was analyzed using MHC-1 binding prediction tool in IEDB to predict T cell epitopes that interacting with different HLA MHC1alleles. The result predicted 91 CTL epitopes that interacted with MHC-I alleles. These epitopes, their positions in glycoprotein D and their interacted alleles were shown in Table 3. Three epitopes namely 24STAAVTYDY32, 20FASQSTAAV28 and 353FAAFVACAV361 demonstrated strong interaction with MHC-I alleles. Thus they were proposed as CTL cells epitopes. The positions of these epitopes at the 3D structural level of glycoprotein D were shown in Figure 4.
The activation of the T-helper subtypes and their corresponding cytokines secretion is one of the important characteristics of immune responses as they are required for almost all adaptive immune responses 36. Peptides binding to MHC class II molecules were evaluated using human alleles. Several methods were used for analysis of MHC-II epitopes binding grooves. However the NN- align is important for instantaneous identification of the MHC class II binding core epitopes 37, 38. Several core peptides were predicted to interact with considerable number of MHCII alleles. Four epitopes namely 88FEASVVWFY96, 212FQGEHLYPI220,, 353FAAFVACAV361 and 137VDYVPSTLV145 exhibited great binding interaction to 82, 71, 62 and 69 alleles to MHC-II, respectively. These epitopes, their positions in glycoprotein D and their interacted alleles were shown in Table 4. Also the positions of these epitopes at the 3D structural level of glycoprotein D were shown in Figure 4 and Figure 5.
In this study several epitopes were predicted to interact with both MHC I and MHC II alleles. As shown in table (5) the three proposed epitopes that strongly interacted with MHC class I alleles were also strongly interacted with MHC II alleles. For instance 20FASQSTAAV28 interacted with 9 and 52 alleles in MHC I and MHC II respectively. The same epitope (20FASQSTAAV28) overlapped with epitope 24STAAVTYDY32 that interacted with 10 and 1 alleles in MHC I and MHC II respectively. Moreover the epitope 353FAAFVACAV361 was interacting with 9 and 37 alleles of MHC I and MHC II respectively. Concerning epitopes proposed for MHC II the best epitope was 88FEASVVWFY96 since it interacted with 7 and 71 alleles in MHCI and MHC II, respectively. Moreover the epitope 212FQGEHLYPI220 that associated with 82 alleles for MHC II was linked to 3 alleles only from MHC I. However the epitope 137VDYVPSTLV145 was found only interacting with MHC II alleles.
Docking is commonly known for its wide application in computer-aided drug design. However, it is also used for designing novel peptides exhibiting binding affinity towards MHC molecules. Originally, the docking studies were mainly used for investigation of peptides that bind MHC class I molecules 8. Molecular docking was performed using peptide-binding groove affinity. This help in the prediction and symbolization of the real image of CTL epitopes interaction (ligands) with chicken alleles (receptors). For this purpose, as shown in Figure 6, two types of chicken BF alleles (BF2*2101 & BF2*0401) were used for docking with the epitopes 24STAAVTYDY32, 20FASQSTAAV28 and 353FAAFVACAV361 that proposed for CTL. The lowest binding energy (kcal/mol) was selected to predict probable CTL epitopes based on the score of global energy and attractive VDW in kcal/mol unit of docked molecules. As shown in Table 6, docking of 353FAAFVACAV361 epitope with BF2 2101 and BF2*0401alleles produced -67.87 and -50.57 global energy respectively. This indicated the strong binding affinity between the ligand and both receptors compared to other epitopes. Moreover the epitopes 24STAAVTYDY and 20FASQSTAAV28 both demonstrated favorable binding affinity with BF2 alleles. The BF2*2101 chicken alleles displayed strong binding affinity with CTL proposed peptides compared to BF2*0401. Moreover, the docked epitopes demonstrated deep binding grooves in both BF alleles. Figure 7 illustrated the deep binding between the BF2 alleles and the docked molecules using Patch Dock server for molecular docking.
Compared to the recent in silico study on glycoprotein B of ILTV similarity and difference were observed in the docked epitopes form glycoprotein D to the chicken alleles 13. For instance epitopes predicted from the glycoprotein B and D of ILTV demonstrated similarity in their high binding affinity of the ligands to the receptor BF2*2101 alleles compared with BF2 0401 alleles. However, the docked molecules from glycoprotein B and D demonstrated different binding sites for BF2*2101 allele and similar binding sites for BF2*0401 allele (Figure 6 and Figure 7). Moreover, the overall binding affinity of glycoprotein D to BF alleles was less compared to that obtained by glycoprptein B 13.
Vaccines evoke profound changes in the cellular components of adaptive immunity, comprising T- and B-cells. Peptide vaccine can induce specific immune responses because it contains immunodominant peptides. These peptides are constructed on the basis of a chemical approach to synthesize the identified B cells and T cells epitopes. B-cell epitope of a target molecule can be linked with a T-cell epitope to make it immunogenic.
The immunoinformatics tools are used in diversity of applications from basic immunological data to computational techniques and assays. For example conduction of potent biomedical research for prediction of new epitopes, vaccines design and design of immune-based. The traditional peptide vaccine is costive and takes long time to produce.
In vitro and in vivo tests are needed to achieve and exemplify the effectiveness of the proposed epitopes to induce an immune response. Peptide vaccine against glycoprotein is strongly supersedes the conventional vaccines, this new universal predicted vaccine for chicken.
Authors would like to thank the staff members of department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Sudan for their cooperation and support.
The authors declared that they have no conflict of interests regarding the publication of this paper.
No funding was received.
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Published with license by Science and Education Publishing, Copyright © 2020 Manahel J. Ibrahim, Sumaia A. Ali, Khoubieb A. Abd-elrahman and Yassir A. Almofti
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[1] | Thapa, S., et al., In ovo delivery of CpG DNA reduces avian infectious laryngotracheitis virus induced mortality and morbidity. Viruses, 2015. 7(4): p. 1832-52. | ||
In article | View Article PubMed | ||
[2] | McGeoch, D.J., F.J. Rixon, and A.J. Davison, Topics in herpesvirus genomics and evolution. Virus research, 2006. 117(1): p. 90-104. | ||
In article | View Article PubMed | ||
[3] | Fahey, K., T. Bagust, and J. York, Laryngotracheitis herpesvirus infection in the chicken: the role of humoral antibody in immunity to a graded challenge infection. Avian Pathology, 1983. 12(4): p. 505-514. | ||
In article | View Article PubMed | ||
[4] | Rodríguez-Avila, A., et al., Replication and transmission of live attenuated infectious laryngotracheitis virus (ILTV) vaccines. Avian diseases, 2007. 51(4): p. 905-911. | ||
In article | View Article PubMed | ||
[5] | García, M., et al., Genomic sequence analysis of the United States infectious laryngotracheitis vaccine strains chicken embryo origin (CEO) and tissue culture origin (TCO). Virology, 2013. 440(1): p. 64-74. | ||
In article | View Article PubMed | ||
[6] | Kingham, B.F., et al., The genome of herpesvirus of turkeys: comparative analysis with Marek’s disease viruses. Journal of General Virology, 2001. 82(5): p. 1123-1135. | ||
In article | View Article PubMed | ||
[7] | Kirkpatrick, N.C., et al., Differentiation of infectious laryngotracheitis virus isolates by restriction fragment length polymorphic analysis of polymerase chain reaction products amplified from multiple genes. Avian diseases, 2006. 50(1): p. 28-33. | ||
In article | View Article PubMed | ||
[8] | Patronov, A. and I. Doytchinova, T-cell epitope vaccine design by immunoinformatics. Open biology, 2013. 3(1): p. 120139. | ||
In article | View Article PubMed | ||
[9] | Reche, P.A., et al., Peptide-based immunotherapeutics and vaccines. Journal of immunology research, 2014. 2014. | ||
In article | View Article PubMed | ||
[10] | Flower, D.R., Designing immunogenic peptides. Nature chemical biology, 2013. 9(12): p. 749-753. | ||
In article | View Article PubMed | ||
[11] | Bande, F., et al., Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for application in peptide vaccines. Advances in bioinformatics, 2016. | ||
In article | View Article PubMed | ||
[12] | Zheng, J., et al., In Silico Analysis of Epitope-Based Vaccine Candidates against Hepatitis B Virus Polymerase Protein. Viruses, 2017. 9(5): p. 112. | ||
In article | View Article PubMed | ||
[13] | Ali, S.A., Y.A. Almofti, and K.A. Abd-elrahman, Immunoinformatics Approach for Multiepitopes Vaccine Prediction against Glycoprotein B of Avian Infectious Laryngotracheitis Virus. Advances in Bioinformatics, 2019. 2019. | ||
In article | View Article PubMed | ||
[14] | National Center for Biotechnology Information (NCBI): https://www.ncbi.nlm.nih.gov/protein/. Accessed 19 Oct. 2018 | ||
In article | |||
[15] | Hall, T., BioEdit: an important software for molecular biology. GERF Bull Biosci, 2011. 2(1): p. 60-61. | ||
In article | |||
[16] | Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6:molecular evolutionary genetics analysis version 6.0.MolBiolEvol. 2013; 30: 2725-2729. | ||
In article | View Article PubMed | ||
[17] | Vita, R., et al., The immune epitope database (IEDB) 3.0. Nucleic acids research, 2014. 43(D1): p. D405-D412. | ||
In article | View Article PubMed | ||
[18] | Larsen, J.E., O. Lund, and M. Nielsen, Improved method for predicting linear B-cell epitopes. Immunome research, 2006. 2(1): p. 2. | ||
In article | View Article PubMed | ||
[19] | Emini, E.A., et al., Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. Journal of virology, 1985. 55(3): p. 836-839. | ||
In article | View Article PubMed | ||
[20] | Kolaskar, A. and P.C. Tongaonkar, A semi-empirical method for prediction of antigenic determinants on protein antigens. FEBS letters, 1990. 276(1-2): p. 172-174. | ||
In article | View Article | ||
[21] | Morshed, M.M., et al., Computer aided prediction and identification of potential epitopes in the receptor binding domain (RBD) of spike (S) glycoprotein of MERS-CoV. Bioinformation, 2014. 10(8): p. 533. | ||
In article | |||
[22] | Nielsen, M. and O. Lund, NN-align. An artificial neural network-based alignment algorithm for MHC class II peptide binding prediction. BMC bioinformatics, 2009. 10(1): p. 296. | ||
In article | View Article PubMed | ||
[23] | Wu, S. and Y. Zhang, MUSTER: improving protein sequence profile-profile alignments by using multiple sources of structure information. Proteins: Structure, Function, and Bioinformatics, 2008. 72(2): p. 547-556. | ||
In article | View Article PubMed | ||
[24] | Källberg, M., et al., Template-based protein structure modeling using the RaptorX web server. Nature protocols, 2012. 7(8): p. 1511. | ||
In article | View Article PubMed | ||
[25] | Peng, J. and J. Xu, RaptorX: exploiting structure information for protein alignment by statistical inference. Proteins: Structure, Function, and Bioinformatics, 2011. 79(S10): p. 161-171. | ||
In article | View Article PubMed | ||
[26] | Peng, J. and J. Xu, A multiple-template approach to protein threading. Proteins: Structure, Function, and Bioinformatics, 2011. 79(6): p. 1930-1939. | ||
In article | View Article PubMed | ||
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