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From
Microbial Community Profiling of Active Oleophilic Bacteria involved in Bioreactor-based Crude-oil Polluted Sediment Treatment
Chioma Blaise Chikere, Amara Ukamaka Okoye, Gideon Chijioke Okpokwasili
Journal of Applied & Environmental Microbiology
.
2016
, 4(1), 1-20 doi:10.12691/jaem-4-1-1
Fig
ure
1a
.
The seven 2.5 L bioreactors (Source: Chikere
et al
. 2012)
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Fig
ure
1b
.
The interior design of the 2.5 l bioreactors (Source: Chikere
et al
. 2012)
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Fig
ure
2a
.
Total (TCHUB) Culturable Hydrocarbon Utilizing counts during the 64-day bioremediation
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Fig
ure
2b
.
Total Culturable Heterotrophic Bacterial (TCHB) counts during the 64-day bioremediation
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Fig
ure
3a
.
Residual concentrations of total petroleum hydrocarbon (TPH) in different treatments during the 64-day bioremediation
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Fig
ure
3b
.
Residual concentrations of Polycyclic aromatic hydrocarbon (PAH) in different treatments during the 64-day bioremediation
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Fig
ure
4
.
Percentage degradation of polycyclic aromatic hydrocarbon (PAH) and total petroleum hydrocarbon (TPH) during the 64-day bioremediation
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Fig
ure
5a
.
Measurement of residual fractions of polycyclic aromatic hydrocarbon (PAH) in all treatments on day 0 of bioremediation
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Fig
ure
5b
.
Measurement of residual fractions of polycyclic aromatic hydrocarbon (PAH) in all treatments on day 32 of bioremediation
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Fig
ure
5c
.
Measurement of residual fractions of polycyclic aromatic hydrocarbon (PAH) in all treatments on day 64 of bioremediation
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Fig
ure 6
a
.
Frequency of occurrence for tentative bacterial isolates on day 0
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Fig
ure
6b
.
Frequency of occurrence for tentative bacterial isolates on day 16
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Fig
ure
6c
.
Frequency of occurrence for tentative bacterial isolates on day 32
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Fig
ure
6d
.
Frequency of occurrence for tentative bacterial isolates on day 64
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Fig
ure
7a
.
Graph showing growth curve optical density (O.D) values of bacteria in Bushnell Haas broth + Okono crude oil for a period of 14 days
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Fig
ure
7b
.
Graph showing growth curve optical density (O.D) values of bacteria in Bushnell Haas broth + Okono crude oil for a period of 14 days
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Fig
ure
7c
. Graph showing growth curve optical density (O.D) values of bacteria in Bushnell Haas broth + Okono crude oil for a period of 14 days
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Fig
ure
8a
.
Gel electrophoresis showing amplified 16S rRNA fragments of bacterial DNA (1-33).
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Fig
ure
8b
.
Gel electrophoresis showing amplified 16S rRNA fragments of bacterial DNA (34-66)
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Fig
ure
8c
.
Gel electrophoresis showing amplified 16S rRNA fragments of bacterial DNA (67-72)
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Fig
ure
9a
.
Gel electrophoresis showing amplified DNA fragments of catechol 2,3- dioxygenases (Lane 1-33)
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Fi
gure
9b
.
Gel electrophoresis showing amplified DNA fragments of catechol 2,3 dioxygenases (Lane 34-39)
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Fig
ure
9c
.
Gel electrophoresis showing amplified DNA fragments of catechol 2,3- dioxygenases (Lane 40-50)
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Fig
ure
10a
.
Gel electrophoresis showing amplified DNA fragments of DEG (Lane 1-28)
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Fig
ure
10b
.
Gel electrophoresis showing amplified DNA fragments of DEG (Lane 29-50, -VE)
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