Decolourisation and Detoxification of Reactive Azo Dyes by Saccharothrix Aerocolonigenes TE5

Rizwana Parvez S.; Uma Maheswari Devi Palempalle

doi:10.12691/jaem-3-2-5

Journal of Applied & Environmental Microbiology

Vol. 3, No. 2, 2015, pp 58-62. doi: 10.12691/jaem-3-2-5 | Research Article

OPEN ACCESS

PEER-REVIEWED

Decolourisation and Detoxification of Reactive Azo Dyes by Saccharothrix Aerocolonigenes TE5

Rizwana Parvez S.¹, Uma Maheswari Devi Palempalle^1,

¹Department of Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India

	Abstract
1.	Introduction
2.	Materials and Methods
3.	Results and Discussion
4.	Conclusion
	Acknowledgement
	References

Abstract

Reactive azo dyes primarily present in the effluent of textile industries are recalcitrant molecules and are highly toxic enough to impart severe adverse effects on vital organs of human or animal system and are difficult to degrade by biological means. The present study is structured to study the decolourization and detoxification potential of Saccharothrix aerocolonigenes strain TE5 isolated from the soil contaminated with textile effluents. The percent of decolourization was calculated under static and shaking conditions by employing UV-VIS Spectroscopy. The effect of pH, temperature, carbon and nitrogen sources on the rate of decolourization was determined. The toxicity of the degraded products was assessed by germination assay.

Keywords: azo dyes, textile effluents, actinomycetes, degradation, phytotoxic analysis

Received May 05, 2015; Revised May 19, 2015; Accepted July 21, 2015

Cite this article:

Rizwana Parvez S., Uma Maheswari Devi Palempalle. Decolourisation and Detoxification of Reactive Azo Dyes by Saccharothrix Aerocolonigenes TE5. Journal of Applied & Environmental Microbiology. Vol. 3, No. 2, 2015, pp 58-62. https://pubs.sciepub.com/jaem/3/2/5

S., Rizwana Parvez, and Uma Maheswari Devi Palempalle. "Decolourisation and Detoxification of Reactive Azo Dyes by Saccharothrix Aerocolonigenes TE5." Journal of Applied & Environmental Microbiology 3.2 (2015): 58-62.

S., R. P. , & Palempalle, U. M. D. (2015). Decolourisation and Detoxification of Reactive Azo Dyes by Saccharothrix Aerocolonigenes TE5. Journal of Applied & Environmental Microbiology, 3(2), 58-62.

S., Rizwana Parvez, and Uma Maheswari Devi Palempalle. "Decolourisation and Detoxification of Reactive Azo Dyes by Saccharothrix Aerocolonigenes TE5." Journal of Applied & Environmental Microbiology 3, no. 2 (2015): 58-62.

Import into BibTeX

Import into EndNote

Import into RefMan

Import into RefWorks

At a glance: Figures

View all figures

123

Prev Next

1. Introduction

The textile industry is rated as one of the most prominent polluting industry among all the industrial sectors based on the volume and composition of dyes released in to the water stream ^{[1, 2]}. Globally, the total organic dye production is found to be more than 100,000 tons/year ^[3]. The textile effluents have notable features with reference to pH profile, amount of dissolved oxygen, organic and inorganic chemical complexes ^[4]. Release of textile dyes in to water bodies causes an unpleasant appearance by imparting colour/odor and also the breakdown products of textile dyes are highly toxic that involves in the process of mutagesis and carcinogenesis ^[5]. During the dying process, a sizable amount of dye is lost in the effluent waste water and the loss was reported of about 10-15% of total dyes ^[6].

The textile dyes are catergorised as azo, cationic, basic, anthraquinone and metal complex dyes based on the nature of their chemical structure ^[7]. Azo dyes, which represent about one-half of all other dyes which are commonly engaged as colouring agents in textile industry. The reactive azo dyes are extensively used in textile industry due to their unique characteristics such as brilliant colour band, diverse colour shades, water–fastness profile and easy application technique with low energy consumption ^[8]. The release of azo dyes into the surrounding environment causes serious damage as they intensely affect the photosynthetic activity of hydrophytes by limiting the light penetration and their breakdown products will lead into acute toxicity of ecosystems. The azo groups are generally connected to aromatic heterocyclic or aliphatic groups and these side chains are necessary for imparting the colour of the dye ^[9]. Azo dyes are resistant to degradation and remains persistent for long time due to their fused aromatic structure ^[10]. By keeping in view the importance of azo dyes, the present study is designed to evaluate the potential of saccharothrix aerocolonigenes strain TE5, a terrestrial actinomycetes, isolated from the soil sample contaminated by textile effluent.

2. Materials and Methods

2.1. Dyes

Azo dyes – namely Reactive Red 1 (RR1), Reactive Orange 107 (RY107) and Reactive black 5 (RB5) are collected from Madanapalle town, Chittoor district, Andhra Pradesh, India.

2.2. Preparation of Dye Stock Solution

The stock solution of the three dyes was prepared by dissolving 2.0g in 10ml distilled water (200 mg/m1) and sterilized by autoclaving at 120°C for 30 minutes.

2.3. Microorganisms and Culture Media

The isolate was designated as Saccharothrix aerocolonigenes strain TE5 based on the biochemical and molecular characterization (data not shown) and was maintained on Glucose malt extract (Glucose 4g;Yeast extract 4g; Malt extract 10g; CaCO3 2g; Distilled water 1000ml) and preserved for further use.

2.4. Decolourisation Assay

Typical decolourization experiments were carried out in 250 ml conical flasks with 100 ml volumes of a sterile Mineral salt medium. Saccharothrix aerocolonigenes strain TE5 was inoculated into mineral medium containing 600 ppm of one of the dye such as RR1, RY107 or RB5 and incubated at 37°C both under static and shaking conditions. Control was maintained without the inoculum. Three independent experiments were conducted for the selected azo dyes. All samples were collected at regular intervals, centrifuged at 10000g for 10 min and the supernatant was used for measuring absorption at 410 ηm for RY-107 and 540ηm for RR1 or RB5 using UV-VIS spectrophotometer. The percent decolourization of reactive azo dyes was determined ^[11].

2.5. Decolourization at Different Temperatures

In order to determine the optimum temperature for the decolourization of the azo dyes, the test tubes with minimal broth were inoculated with 0.1 ml of overnight cultures and incubated at different temperatures ranging from 4°C to 45°C for 48h. At the end of incubation period, the magnitude of decolourization of the reactive dyes was measured and optimum temperature was recorded.

2.6. Effect of pH on Decolourisation

The ability of the isolate to decolourise the azo dyes at pH ranging from 3 – 9 was tested in minimal broth by keeping other conditions as constant (dye concentration 600 mg/L, temperature 37°C. The percent of decolourization was measured after 24 h.

2.7. Effect of Carbon on Decolourisation

The Mineral Salt Medium containing 600 ppm of dye was supplemented with carbon sources (1%) such Glucose, Lactose and Sucrose to determine their effect on the degradation potential of Saccharothrix aerocolonigenes strain TE5. The experiments with individual dye were performed at pH 7 and temperature 37°C for 24h.

2.8. Effect of Nitrogen on Decolourization

To determine the effect of Nitrogen sources on decolourisation, the medium was augmented with 1% Nitrogen sources (Peptone, Beef extract and Yeast Extract) and was inoculated with Saccharothrix aerocolonigenes strain TE5 at 37°C for 24h. At the end of incubation, the percent of decolurisation was estimated.

2.9. Bioassy for Phytotoxicity

The phytotoxic nature of the degraded azo dye was studied by seed germination assay. The tolerance level of the wheat and green gram towards the toxicity of the RR1dye and RY107 respectively and their degraded products were observed. The Saccharothrix aerocolonigenes TE5 culture was inoculated to the Mineral Salt Medium containing 600ppm of Dye RR1 and incubated at 37°C for 48h. After degradation of the dye, the culture was harvested and centrifuged at 10,000 rpm for 10 minutes and supernatant was collected. The seeds of both wheat and green gram were soaked either in the decolourised supernatant or original dye (600 mg/l). The seeds wet with the distilled water were taken as the control. After overnight period, the soaked seeds were placed on the paper towels for germination ^[12]. Germination was continuously monitored for 7days. The perncentage of germination, the length of plumule and radical of the germinated seeds was measured after 7days and the results were tabulated.

2.10. Statistical Analysis

The results obtained during experimentation were expressed in terms of Means and Standard Error (SE). Data was defined statistically by ANOVA (Single factor), T-test. Correlation between different parameters was calculated by SPSS software. Probability (p-value) less than 0.05 and 0.01 was considered significant and highly significant respectively.

3. Results and Discussion

The Saccharothrix aerocolonigenes TE5 was employed for the degradation of the three reactive azo dyes. From the present study, it was found that Saccharothrix aerocolonigenes TE5 could decolourize the reactive azo dyes significantly within 48h. The efficacy of decolourisation depends upon the adaptability and survival of these microorganisms during the degradation processes ^[13]. As shown in Figure 1, the Saccharothrix aerocolonigenes TE5 decolourized RR1 (92%), RY 107 (90%) and RB (54%) from the MSM medium after 48h of incubation under static conditions whereas, the percentage of decolourization was reduced from 92% to 62% for RR1, from 90% to 74% for RY107 and from 54% to 41% for RB5 under shaking conditions (Figure 2).

Download as

Veiw figureFigures index

Figure 1. Decolourisation of Reactive azo dyes by Saccarothrix aerocolonigenes TE5 under static conditions. The data indicate the mean of three independent experiments Mean±SEM. p<0.001

Initially, the effect of pH on the decolourization of reactive dyes was studied at a pH range of 3.0–9.0. The Saccharothrix aerocolonigenes TE5 was capable of decolorizing all the three reactive dyes and it showed maximum decolourisation of 92% for RR1 and 80% for RY107 and for 52% with RB5 at pH 7.0 (Figure 3). The degradation percentage was observed to be higher with RY 107 when compared with RR1 and RB5. The optimum pH for decolourization was found to be 7.0 and decrease in decolourisation was noticed both at acidic as well as basic pH range. The enzymatic reduction of the azo bond resulted into the formation of aromatic amines which in turn increases the pH of the medium while comparing with original azo compound ^[14]. This indicates the absence of aromatic amines in the degraded samples of these three dyes. As per the data shown in Figure 4, the optimum temperature was found to be 37°C for effective decolourisation of the selected reactive dyes. The decline in colour removal activity at higher temperatures can be attributed to the loss of cell viability or due to the denaturation of an azo reductase or laccase enzyme ^{[15, 16]}.

Download as

Veiw figureFigures index

Figure 2. Decolourisation of Reactive azo dyes by Saccarothrix aerocolonigenes TE5 under shaking conditions. The data indicate the mean of three independent experiments Mean±SEM. p<0.05

Download as

Veiw figureFigures index

Figure 3. The effect of pH on the decolurization of of three reactive azo dyes by Saccarothrix aerocolonigenes TE5. The data indicates the mean of three independent experiments Mean±SEM. p<0.01

Download as

Veiw figureFigures index

Figure 4. The effect of temperatue on the decolurization of of three reactive azo dyes by Saccarothrix aerocolonigenes TE5. The data indicates the mean of three independent experiments Mean±SEM. p<0.01

Download as

Veiw figureFigures index

Figure 5. The effect of Carbon sources on the colour removal of three reactive azo dyes by Saccarothrix aerocolonigenes TE5. The data indicate the mean of three independent experiments Mean±SEM. p<0.01

Download as

Veiw figureFigures index

Figure 6. The effect of Nitrogen sources on the colour removal of three reactive azo dyes by Saccarothrix aerocolonigenes TE5. The data indicate the mean of three independent experiments Mean±SEM. p<0.01

The decolourization of reactive azo dyes was studied in the presence of carbon and nitrogen additives. The carbon sources such as glucose, fructose and sucrose at 1% were used as co-substrates to investigate their effects on dye decolourization after 24h of incubation with Saccharothrix aerocolonigenes TE5. The culture media supplemented with additional source of glucose exhibited two fold increase in the decolourisation of all three reactive dyes on comparison with sucrose and lactose (Figure 5). The literature reported the correlative influence of glucose concentration on the decolorization of various dyes ^[17]. The effect of different nitrogen sources on the decolourization of selected reactive azo dyes was studied by addition of 1% nitrogen source to Mineral salt medium and incubating with Saccharothrix aerocolonigenes TE5 for 24h. The medium supplemented with beef extract, yeast extract and peptone showed significant effect on the degradation of the dyes RR1, RY107 and RB5. Among nitrogen sources, the degradation was observed to be high with yeast extract than other nitrogen sources (Figure 6). The decolourization of reactive brilliant blue by Rhodocyclus gelatinosus XL-1 significantly increased with the addition of peptone to the reaction medium.

Seed germination assay and plant growth bioassay are the most common methods used to evaluate the toxic nature of the decolourised dyes ^[18]. Thus, in the present study, the phytotoxicity of the dye and its metabolites after degradation by Saccharothrix aerocolonigenes TE5 was observed (Table 1).

Table 1. Effect of degraded and parent RR1 dye on the germination of wheat and bean seedlings

Download as

Veiw tableTables index

NEW
View table in a new window

The seedlings of Triticum aestivum showed 3.5 fold (70%) germination rate with degraded RR1sample on comparison with RR1 control dye (20%) (Figure 7).

Download as

Veiw figureFigures index

Figure 7. The effect of degraded and control RR1 dye on germination of wheat seedlings. The data indicates the mean of three independent experiments Mean±SEM. p<0.01

Download as

Veiw figureFigures index

Figure 8. The effect of degraded and control RR1 dye on length of (a) plumule (b) Radicle

Similarly, Phaseolus aureus seeds with degraded RY107 sample showed significant germination i.e. 3 fold rise (Figure 8). The length of plumule and radicle was observed to be significantly affected by both RR1 and RY107 parent azo dyes than their degradative metabolites, indicating less toxic nature of degradation metabolites as compared with parent dyes. The phytotoxic evaluation studies conducted on the germination and early seedling growth of clover, wheat, lettuce and tomato plants showed similar effects ^{[19, 20]}.

4. Conclusion

Saccharothrix aerocolonigenes TE5 isolated from the contaminated site is potential for the decolourisation of reactive azo dyes namely RR1, RY107 and RB5. The phytotoxicity studies also revealed that the biodegradation of reactive dyes results into the formation of non-toxic metabolites.

Acknowledgement

The authors are very grateful to Government of India sponsored DST-FIST & CURIE Sri Padmavathi Mahila Visvavidyalayam (S.P. Women’s University), Tirupati-517502, Andhra Pradesh, India for providing Research facilities for successful execution of above research.

References

[1]	Vilaseca, M., Gutie, M.C, Grimau, V.L., Mesas, M.L. and Crespi, M., “Biological Treatment of a Textile Effluent After Electrochemical Oxidation of Reactive Dyes,” Water Environ. Res. 82. 176-181. 2010.
	In article	View Article PubMed

[2]	Awomeso, J.A., Taiwo, A.M., Gbadebo, A.M. and Adenowo, J.A., ”Studies on the pollution of waterbody by textile industry effluents in Lagos, Nigeria,” J. Appl. Sci. Environ.Sanit. Sby. 5. 353-359. 2010.
	In article

[3]	Revenkar, S.M. and Lele, S.S., “Synthetic dye decolourization by white rot fungus, Gandoerma sp. WR-I,” Biresour. Technol. 98. 77-780. 2007.
	In article

[4]	Easton, J.R., “The dye maker’s view. In: Cooper P (ed) Color in dyehouse effluent,” Society of Dyers and Colorists, Bradford. 9-21. 1995.
	In article

[5]	Conneely, A., Smyth, W. F. and McMullan, G., “Metabolism of pthalocyanin textilel dye remazol turquoise blue phenerochaete chrysosporium,” FEMS Microbiol Lett. 179. 333-337. 1999.
	In article	View Article PubMed

[6]	Zollinger, H., Color Chemistry-Synthesis, Properties and Application of Organic Dyes and Pigment. VCH Publishers, New York. 1987, 92-102.
	In article

[7]	McMullan, G., Meehan, C., Conneely, A., Kirby, N., Robinson, T., Nigam, P., Banat, I.M., Marchant, R. and Smyth, W.F., “Microbial decolorization and degradation of textile dyes: Mini Review,” Appl. Microbiol. Biotechnol. 56. 81-87. 2001.
	In article	View Article PubMed

[8]	Rajeshwari, K., Subashkumar, R. and Vijayaraman, K., “Biodegradation of mixed textile dyes by bacterial strain isolated from dye waste effluent,” Res. J. of Env. Toxicology, 5(2). 97-107. 2011.
	In article	View Article

[9]	Mc Curdy, M.W., Boardman, G.D., Michelsen, D.L. and Woodby, B.M., “Chemical reduction and oxidation combined with biodegradation for the treatment of textile dye,” in 46^th proc. purdue Industrial waste conf., Lewis publishers, MI, 229-234. 1992.
	In article

[10]	Xu, Meiying, J., Guo, G., Zeng, X., Zhong and Sun, G., “Decolourization of anthraquinone dye by Shewanella decolouriations S12,” Appl. Microbiol. Biotechnol. 71. 246-251. 2006.
	In article	View Article PubMed

[11]	Rizwana Parvez, S. and Uma Maheswari Devi, P., “Decolourization of Reactive azo dyes by Aspergillus niger from dying industry effluent” International Journal of Scientific & Engineering Research, 6. (2). 45-49. 2015.
	In article

[12]	Sujatha, B., Rashmi, H.K. and Uma Maheswari Devi, P., “Antifungal Activity of Oxylipins Against Papaya Fungal Pathogens,” Journal of Experimental Biology and Agricultural Sciences, 1 (2S). 139-145. June – 2013.
	In article

[13]	Chang, J.S., Chou, Y.P. and Chen, Y.S., “Decolourization of azo dyes with immobilized Pseudomonas luteola,” Process Biochem, 36. 757-763. 2001a.
	In article	View Article

[14]	Willmott, N., Guthrie, J. and Nelson, G.,”The biotechnology approach to colour removal from textile effluent,” J. Soct. of Dyers and Colourists. 114. 38-41. 1998.
	In article	View Article

[15]	Telke, A., Kalyani, D., Jadhav, J. and Govindwar, S., “Kinetics and mechanism of reactive Red 141 degradation by a bacterial isolate Rhizobium radiobacter MTCC 8161,” Acta Chim. Slov, 55. 320-329. 2008.
	In article

[16]	Saratale, R.G., Saratale, G.D., Kalyani, D.C., Chang, J.S. and Govindwar, S.P., “Enhanced decolorization and biodegradation of textile azo dye scarlet R by using developed microbial consortium-GR,” Bioresour. Technol., 100. 2493-2500. 2009b.
	In article	View Article PubMed

[17]	Jadhav, S.U., Jadhav, M.U. Kagalkar, A.N. and Govindwar, S.P., ”Decolourization of briliant blue G Dye mediated by degradation of the microbial consortium of Galactomyces geotrichum and Bacilus sp.,” J. Chin. Inst. Chem. Engrs., 39. 563. 2008.
	In article	View Article

[18]	Mbuligwe, S.E., “Comparative treatment of dye-rich wastewater in engineered wetland systems (EWSs) vegetated with different plants,” Water Research, 39. 271-280, 2005.
	In article	View Article PubMed

[19]	Oliveira, D.P., Carneiro, P.A., Sakagami, M.K., Zanoni, M.V.B. and Umbuzeiro, G.A., “Chemical characterization of a dye processing plant effluent – Identification of the mutagenic components,” Mutation Research, 626. 135-142. 2007.
	In article	View Article PubMed

[20]	Saratale, R.G., Saratale, G.D., Kalyani, D.C., Chang, J.S. and Govindawar, S.P., “Enhanced decolorization and biodegradation of textile azo dye Scarlet R by using developed microbial consortium-GR,” Bioresource Technology, 100. 2493-2500. 2009.
	In article	View Article PubMed