Figure 1. Alignment of OTU specific primers with target 16S and ITS sequences. Target regions are highlighted with black boxes. Primer sites and directionality are outlined with red bars in each figure. OTU names are the same as those in Debenport et al. (2015). Primers designed for clusters in A) Chitinophaga, B) Aspergillus, C) Fusarium, D) Lasiodiplodia, and E) Penicillium genera

From

Independent Validation of Differential Abundance Patterns from Illumina Miseq Analysis Using Quantitative PCR Techniques on the Selective Primer for Chitinophaga

Spencer Debenport, Laura Mason, Richard P Dick

Journal of Applied & Environmental Microbiology. 2023, 11(1), 1-10 doi:10.12691/jaem-11-1-1