Figures index

From

Independent Validation of Differential Abundance Patterns from Illumina Miseq Analysis Using Quantitative PCR Techniques on the Selective Primer for Chitinophaga

Spencer Debenport, Laura Mason, Richard P Dick

Journal of Applied & Environmental Microbiology. 2023, 11(1), 1-10 doi:10.12691/jaem-11-1-1
  • Figure 1. Alignment of OTU specific primers with target 16S and ITS sequences. Target regions are highlighted with black boxes. Primer sites and directionality are outlined with red bars in each figure. OTU names are the same as those in Debenport et al. (2015). Primers designed for clusters in A) Chitinophaga, B) Aspergillus, C) Fusarium, D) Lasiodiplodia, and E) Penicillium genera
  • Figure 2. Specificity of OTU cluster-specific primer sets. Primer names are provided above each group of PCR products. Within each group of PCR products, the first four lanes use DNA template from intercropped millet samples and the last four lanes are from millet grown in bare soil. 100Bp ladder used
  • Figure 3. Specificity of Chitinophaga primer set. First four lanes use DNA template from intercropped millet samples. Last four lanes use DNA template from millet grown in bare soil. Bands from intercropped millet samples are 130% brighter on average. 100bp ladder used
  • Figure 4. Quantification of Chitinophaga markers in millet root zone soils. Low CT (threshold cycle) and higher LogSQ (log starting DNA quantity) scores indicated higher abundance of molecular targets in sample template DNA. Intercropped millet samples are in blue, and millet grown in bare soil samples are in red