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From

Production of Polyhydroxyalkanoate (PHA) by a Moderately Halotolerant Bacterium Klebsiella pneumoniae U1 Isolated from Rubber Plantation Area

Urvija Apparao, Veena Gayathri Krishnaswamy

International Journal of Environmental Bioremediation & Biodegradation. 2015, 3(2), 54-61 doi:10.12691/ijebb-3-2-3
  • Figure 1. Enriched Bacterial isolate (U1) on Nitrogen-deficient Medium
  • Figure 2. PHA granules in Bacterial strain U1 by Sudan Black Staining
  • Figure 3. PHA granules observed as bright orange granules at 460 nm
  • Figure 4. Extracted PHA after purification and drying
  • Figure 5. (a) PHA produced by the strain U1, (b) Total Protein content of the strain U1
  • Figure 6. UV Spectrum -Absorption maxima of a) Reference Crotonic acid (Standard) b) PHA converted to crotonic acid (Test)
  • Figure 7. FTIR chart showing characteristic peaks of PHA produced by the isolate U1
  • Figure 8. a) PHA production by the isolated strain U1, b) Total Protein content of strain U1 in various carbon sources
  • Figure 9. a) PHA production by the bacterial strain U1, b) Total Protein content of isolate U1 in various nitrogen sources
  • Figure 10. a) PHA production by the strain U1, b) Protein content of strain U1 at different NaCl concentrations
  • Figure 11. Average yield of PHA by the isolate U1 in various NaCl concentrations
  • Figure 12. Phylogenetic analysis of Klebsiella pneumoniae subsp. pneumoniae strain MGH78578 (U1) with its nearby substrains. The numbers at the nodes represent bootstrap values (500 replicates)