Figure 9. Photomicrographs shows the morphological features of the differentiated neural-like cells: (a)Neural precursor cell stained with H&E, with elongated neuriteemerged from the soma after 24hrs(Scale bar = 50µm). (b)Semi-differentiated neurons stained with H&E after 5 daysof induction,with distinct soma, axonand distinguished multi-branched dendrites emerge from it(Scale bar = 50µm).(c)Fully differentiated mature astrocytes after 5 daysof induction stained with H&E,with distinct soma and multi-branched dendrites (Scale bar = 50µm).(d) Neuronal precursor-like cell examined with SEMafter 24 hrsof induction, has pyramidal or cone-like structure soma with central rounded nucleus and extending neurite (Scale bar = 10µm). (e)Mature multipolar neuron (red arrow) after 5daysof induction, examined with SEM, has distinct soma with clearly appeared central rounded nucleus, distinct axonand multi-branched dendrites (Scale bar = 50µm). (f)After 9 days of induction,a very elongated bipolar neuron cell observed using SEM (Scale bar = 50µm)

From

Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into Neural Cells via Cerebrospinal Fluid

Dina Y. Otify, EmanA. Youssef, Naglaa B. Nagy, Mona K. Marei, Magda I. Youssif

Biomedicine and Biotechnology. 2014, 2(4), 66-79 doi:10.12691/bb-2-4-2