Figure 6. Photomicrographs by IPCM showing the morphological features of the differentiated neural-like cells: (a) Neuronal precursor cell has pyramidal soma with rounded nucleus and elongated neurite (24 hrs after induction)(Scale bar = 100µm).(b) More developed immature neuronal cells possess two to three short neurites after 3 days of inductionin CSF(Scale bar = 100µm). (c) Completely developed maturemultipolarneuron with distinct soma and distinct axon and distinguished multi-branched dendrites emanated from it after 5 days of induction.(d)Mature "bipolar" neuron developed within 9 days of induction(Scale bar = 100µm). (e) Immature Astrocyte cell has polygonal cell body with rounded nucleus and more processes emerged from it within 3 days of incubation (Scale bar = 100µm). (f) Mature astrocyte completely differentiated after 5 days of induction, with distinct star like structure soma and dense plasma to fibrous dendrites(Scale bar = 100µm)

From

Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into Neural Cells via Cerebrospinal Fluid

Dina Y. Otify, EmanA. Youssef, Naglaa B. Nagy, Mona K. Marei, Magda I. Youssif

Biomedicine and Biotechnology. 2014, 2(4), 66-79 doi:10.12691/bb-2-4-2