Figure 5. Photomicrographs showing the characterization of neural progenitor-like cells differentiated from BM-MSCs after 4 days ofincubation in culture media supplemented with CSF: (a) Differentiated neural progenitor cells stained strongly with silver impregnation stain (Scale bar = 200µm). (b, c) Higher magnification to the previous showing differentiated multipolar (red arrows) and bipolar (white arrow) neuronal progenitor cells (Scale bar = 50µm).(d) Immunofluorescence image showing differentiated neuronal progenitor cells expressed positive GFAP immunoreactivity culturing (Scale bar = 50µm).(e) Immunofluorescence image showing differentiated neuronal progenitor cells attached with pro-oligodendrocyte precursor cell expressed positive GFAP immunoreactivity (Scale bar = 50µm)

From

Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into Neural Cells via Cerebrospinal Fluid

Dina Y. Otify, EmanA. Youssef, Naglaa B. Nagy, Mona K. Marei, Magda I. Youssif

Biomedicine and Biotechnology. 2014, 2(4), 66-79 doi:10.12691/bb-2-4-2