Figure 4. Photomicrographs shows proliferation and differentiation of neural progenitor-like cells from BM-MSCs after incubation in culture media supplemented with CSF: (a) Phase-contrast microscopy picture showing differentiated bipolar neuronal progenitor cells (red arrows) after 4 days, yellow arrow indicate pro-oligodendrocyte precursor cell (Scale bar = 100µm). (b) Phase-contrast microscopy picture showing formation of connections between branched oligodendrocyte progenitor cell (yellow arrow) and attached with them oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells (Orang arrow) (Scale bar = 100µm). (c, c’) Phase-contrast microscopy picture showing differentiated multipolar neuronal progenitor cells (red arrow) after 4 days, orang arrows indicate multinucleated neural progenitors (stem cells) differentiated into oligodendrocyte-type-2 astrocyte (O-2A)-like progenitor cells (Scale bar = 100µm). (d, d’) Phase-contrast microscopy pictures showing neural elongated-like stem cell after 7 days (Scale bar = 100µm)

From

Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into Neural Cells via Cerebrospinal Fluid

Dina Y. Otify, EmanA. Youssef, Naglaa B. Nagy, Mona K. Marei, Magda I. Youssif

Biomedicine and Biotechnology. 2014, 2(4), 66-79 doi:10.12691/bb-2-4-2