Figure 11. characterization of mature neural-like cells differentiated from BM-MSCs after incubation in culture media supplemented with CSF using GFAP Immunolabelinginvestigated by CLSM: (a)Negative GFAP expression in the undifferentiated BM-MSCs before the differentiation process (0 day)(Scale bar = 50µm).(b)Immunofluorescence image showing positive GFAP immunolabeling for astrocytes within 3 days of incubation,investigated by CLSM(Scale bar = 50µm).(c)Strong GFAP immunolabeling for pseudo-unipolar neuronal-like cell after 5 days, investigated by CLSM(Scale bar = 50µm).(d, d')Mature unipolar neuronal-like cell showing positive GFAP immunoreactive and silver stain, respectively (Scale bar = 50µm).(e, f) Immunofluorescence image showing mature neuronal and oligodendrocyte cells GFAP immunoreactive (Scale bar = 50µm). (e’, f’) Inverted phase micrograph of e) and f) showing perineural oligodendrocytes; Oligodendrocyte (red arrow), Neuronal cells (yellow arrow). (Scale bar = 50µm)

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Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into Neural Cells via Cerebrospinal Fluid

Dina Y. Otify, EmanA. Youssef, Naglaa B. Nagy, Mona K. Marei, Magda I. Youssif

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