Figure 10. Phase-contrast microscopy picture showing cytological characterization of mature neural-like cells differentiated from BM-MSCs after incubation in culture media supplemented with CSF using special stains: (a) Before the induction process (0 day), the cultured cells appeared normally as spindle shape with weak reaction activity and clearly stained nuclei(Scale bar = 50µm).(b) Differentiated neural-like cells stained with cresyl violet after 5 days of induction showing strong concentration of Nissl bodies "NB" granules surrounded the nucleus "N"(Scale bar = 50µm).(c) Fully differentiated mature neuronal-like cells after 9 days of induction, having soma counterstained with cresyl violet (red arrow)and multi-branched neurits impregnated with black silver stain (blue arrow), forming fibrous network-like structure (red square)(Scale bar = 200µm).(d, e)Higher magnification of the neuron-like cells and fibrous neural-like network in the previous figure (Scale bar = 50μm).(f)Fully differentiated mature neuronal-like cells after 14 days, forming fibrous network-like network (red square) (Scale bar = 200µm).(g)Very dense fiberousneuronal network shown by SEM (Scale bar = 5μm).(h) Undifferentiated BM-MSCs before the induction process (0 day), showing spindle shaped cells with very weak PASstain in their cytoplasm(Scale bar = 50µm).(i, j)Mature multipolar neuronal-like cell stained with PAS showing strong glycogen activity, and stronglystained withcresyl violet, respectively(Scale bar = 50µm).(k)Mature bipolar neuron performed after 7 daysof induction which possess distinct soma with strong glycogen content(Scale bar = 50µm).(l) Mature astrocyte-like cells stained with PAS after 5 days of induction in CSF, revealedintense glycogen content(Scale bar = 50µm) : Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into Neural Cells via Cerebrospinal Fluid : Science and Education Publishing