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From

In vitro Anti-inflammatory and in vivo Immuno-modulatory Activities of Ethanolic Extract of Strychnos camptoneura (Loganiaceae) Seeds

Morabandza Cyr Jonas, Moulari Brice, Gombé Assoungou Hermann, Abena Ange Antoine

American Journal of Pharmacological Sciences. 2022, 10(1), 38-46 doi:10.12691/ajps-10-1-7
  • Figure 1. Seeds ethanolic extract of S. camptoneura (EE) solutions of different concentrations were tested for their cytotoxicity to HaCaT cells (A), dermal fibroblast cells (B) after incubation for 8 h. IC50 values were determined using the graph (C). Data are shown as mean±S.D
  • Figure 2. LPS solutions of different concentrations were tested for their cytotoxicity to HaCaT cells (A), dermal fibroblast cells (B) after incubation for 8 h. (C) and (D) represent the TNFα and IL-1β secretion after cell activation during 4 h by LPS. Data are shown as mean±S.D. *p<0.05 compared with non-activated cells (concentration 0)
  • Figure 3. TNFα (A, B) and IL-1β (C, D) secretions of non-activated (healthy) and activated keratinocytes and fibroblast cells after treatment with different concentrations EE at different incubation time; data given as mean±S.D., *p<0.05 compared with LPS control; *p<0.05 compared with EE at 0.75 μg/ml
  • Figure 4. Flow cytometric analysis of blood immune cells in the animals of the non-treated, positive controls, and EE-treated groups in percentage. Myeloid cells were identified based on the expression of CD11b CD11c and F4/80; lymphocyte cells were identified based on the expression of CD3, CD4, CD8 and NK (natural killer). Data are shown as mean ± SD for n = 6 animals per group. *p< 0.05 compared with non-treated control, £p< 0.05 compared with EE-treated group. ANOVA followed by Dunn’s test was used for all pairwise multiple comparisons
  • Figure 5. pictures of Flow cytometric analysis of blood immune cells in the animals of the non-treated, positive controls, and EE-treated groups. Myeloid cells were identified based on the expression of CD11b CD11c and F4/80; lymphocyte cells were identified based on the expression of CD3, CD4, CD8 and NK (natural killer)
  • Figure 6. Effects of EE in circulating anti-inflammatory cytokines (IL-4 and IL-10).*p < 0.05 compared with non-treated group