1. Introduction

An Automated Teller Machine (ATM) is a computerized telecommunications device that enables the clients of a financial institution to perform financial transactions without the need for cashier, human clerk or bank teller. ATMs are known by various other names including ATM machine, automated banking machine, cash dispenser and various regional variants derived from trademarks on ATM systems held by particular banks ^[28]. Automated Teller machine are the most widely used form of computer driven public technology ^[18] with an estimated over 2.4 million units in use ^[1] since their invention and use in the late 1960’s. A typical usage of the ATM machine involves slotting a card into a recipient hole and following on screen instructions, by punching the keys of the metallic keypads to enter secret codes and commands; thus instructing the machine as to kind of service one requires ^[2].

Contamination of environmental objects and surfaces is a common phenomenon. Human beings have a marked tendency to pick up microorganisms from environmental objects, and the hand has been shown to play a role in the transmission of organisms. The ATM machine is likely to be contaminated with various microorganisms due to their vast contact by multiple users. There is no restriction as to who has access to the facility and no guideline to ensure hygienic usage. But like all surfaces microbial colonization of these metallic keypads are eminent, particularly when there are no proper cleaning regimens in place for most of these facilities.

The presence of viable pathogenic bacteria on inanimate objects has been reported by earlier investigators ^{[9, 13, 14, 24]}. Salmonella Species and Escherichia coli strains has also been shown to be transferred from hands to raw processed and cooked foods, even at low levels on the fingers ^{[19, 30]}. Furthermore, microorganisms found to contaminate formites has also been shown to persist on environmental surfaces in varying periods of time ranging from hours to months ^[12].

In addition, cross infection of microorganisms between environmental surfaces and a host has equally been established ^[16]. It has also been shown that microbes once attached to hands and some surfaces may survive for a while and may be difficult to remove ^{[10, 17]}. Many factors have been shown to influence the bacteria transfer between surfaces, including the source and destination surface features, bacterial species involved, moisture levels, pressure and friction between the contact surfaces ^{[7, 22, 30, 35]}.

While the contamination of banks ATMs by microorganisms has been established, most of the previous study has been concentrated within Abakaliki, the capital city of Ebonyi State, or such study was specific to the isolation of a particular microorganism. In view of this, the present study was undertaken to investigate probable bacterial contamination and antibiogram of bacteria present on the touched metallic keypads of Banks ATMs within the two major towns in Ebonyi State (Abakaliki and Afikpo) were these machines are in use.

2. Materials and Methods

This study was carried out in two major towns within Ebonyi State (Afikpo and Abakaliki). The study was undertaken between June to December, 2013.

ATMs of seventeen different banks namely: First bank, Zenith bank, Eco bank, Fidelity bank, Enterprise bank, United Bank for Africa (UBA), Diamond bank, FCMB and Keystone bank, situated in Ebonyi State was used for the study. Permission was sought from the management of all these banks to use their facilities.

Cotton Swabs made on applicator stick was slightly moistened with sterilized distilled water. One milliliter aliquots of each nutrient broth culture from swabs was pour-plated on nutrient agar, diagnostic sensitivity test agar and CLED agar, all was incubated at 37°C. Colonies from nutrient agar plates was also tested for faecal coliforms in MacConkey broth with Durham tubes ^[33]. Representative colonies was sub-cultured repeatedly on fresh media until pure isolates was obtained.

The isolates were identified, using their colonial and cellular morphology, gram reaction and conventional biochemical identification procedures (Cheesbruogh, 2006).

The antibiotic susceptibility patterns of the isolates to 16 antibiotics were determined by the modified Kirby-Bauer diffusion technique. The concentration of the Standard antibiotics used were Ceftriaxone (30µg), Ampiclox (30µg) Cefixine (10µg), Levofloxacin (10µg), Norfloxacin (10µg), Ciprofloxacin (10µg), Gentamicin (10µg), Oflaxacin (10µg), Clindamycin (30µg), Erythromycin (30µg), Chloramphenicol (10µg), Ampicillin (30µg), Nitrofuratoin (100µg), Clarithomycin (30µg), Pefloxacin (10µg), Augumentin (30µg).

Standardized culture of each isolate was prepared by picking two distinct colonies using a sterile wire-loop and emulsifying them in 3 ml of physiological saline in a sample bottle. This suspension was then incubated at 37°C overnight. The standardized overnight culture of each isolate was used to flood the surface of Mueller-Hinton agar plates. The excess was drained off and the plates allowed to dry. The standard antibiotic disc was then aseptically placed at reasonable equidistance on the inoculated Muller-Hinton agar plate and the plate allowed standing on the bench for 1 hour. The plates were then incubated at 37°C for 18 hrs. The diameter of the zone of inhibition produced by each antibiotic disc was measured and recorded in millimeters ^[23].

3. Results

The results obtained from both the morphological and biochemical test carried out on the isolates obtained from the ATM machine showed six different bacteria isolates: Staphylococcus aureus, Coagulase negative staphylococcus (CNS), Escherichia coli, Streptocccus Specie, Pseudomonas aeruginosa and Enterobacter specie. The distribution pattern of the bacterial isolates between the two major towns in Ebonyi State shows that a total of 14 bacterial isolates were obtained from the ATMs sampled, and out of the 14 isolates, 3 (21.4%) were obtained from ATMs from Afikpo, while 11 (78.6%) were from ATM from Abakaliki metropolis.

From Table 1, it was observed that Staphylococcus aureus was the commonest organism isolated (28.57%) followed by E. coli and Coagulase negative Staphylococcus with (21.43%) respectively. Pseudomonas aeruginosa and Enterobacter species showed the least percentage occurrence with (7.14%) respectively.

The susceptibility pattern of the isolates to some prescribed antibiotic show that S. aureus and CNS showed the highest susceptibility (25% and 18.75% respectively) to the applied antibiotics, while all the other bacteria showed varied degrees of resistance to the antibiotics tested (Table 2).

Table 1. Percentage distribution of bacterial contaminants on the interfaces of different bank ATMs

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Table 2. Susceptibility of bacterial isolates from A TMs interfaces to commonly used antibiotics

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4. Discussion

In our study an attempt was made to study probable bacterial pathogens that are present in ATM centres in the two major towns of Ebonyi State. The study was conducted to create awareness to the public and users of ATM machines on the possible diseases that are likely to be contacted due to the presence of pathogen at ATM Centres. The result of this study showed bacterial contaminations on the surfaces of the metallic keypads of ATMs in Ebonyi State. A total of six bacterial isolates were recovered from ATM interface in this study. Qualitative analysis of the bacterial isolates revealed the abundance of skin flora belonging to Coagulase - negative Staphylococcus (CNS), Staphylococcus aureus, Escherichia coli, Streptococcus spp, Pseudomonas aeruginosa, and Enterobacter spp. The percentage distribution of the bacterial isolates showed that Staphylococcus aureus was the commonest isolate with percentage occurrence of 28.57%, CNS, 21.43%; Streptococcus specie, 14.29%, Pseudomonas aeruginosa, 7.14%, Enterobacter spp, 7.14% and E. coli, 21.43% (Table 1).

The health risks associated with the majority of these bacteria are well documented ^[26]. The bacterial contaminants seen in this study is similar to bacteria that have been recovered from surfaces and objects by other investigators. ^[25] reported that keypads of ATMs harbored more bacteria than computer keyboards and this may be due to the fact that ATMs are usually located in the open, exposed to wind and rain. In addition to the bacteria isolated in our study, ^[25] isolated Klebsiella pneumonia, Proteus spp, Aeromonas viridians, Bacillus spp among others from Electronic hardware interfaces in Ile-Ife. Our study is also in agreement with the study by ^[3] who reported the presence of Staphylococcus Species, Escherichia Species and Klebsiella Species on the keypads of ATM machines. Also high rates of microbial contamination were found on the mobile phones and computer’s keypad which has similar features with ATMs according to their physical and operational aspects. ^[34] reported that cell phones of patients, visitors and Health care workers carried multidrug – resistant hospital pathogens including Acinetobacter spp, S. aureus and extend-spectrum β-lactamase ESBL-positive Enterobacteriaceae. ^{[4, 5]} reported that Staphylococcus aureus were more prevalent on computer keypad and mouse. Interestingly, our study revealed similar result where S. aureus showed the highest prevalence among other isolates. S. aureus is the major component of the normal flora of the skin and nostril, which probably explains its high prevalence as contaminant, and can be easily be discharged by several human activities, like sneezing, talking and contact with moist skin ^[20].

Escherichia coli are the second commonest isolates recovered in our study. E. coli is an enteric pathogen that spread diseases through touch, improper sanitary activities of individuals. Transient pathogens are excreted in feces, various body fluids or tissues by persons infected or colonized by these pathogens. E. coli can cause diseases like gastroenteritis, urinary tract infection, septicemia, dysentery, vomiting, stomach cramps and flatulence.

Other bacteria like Enterobacter spp and Pseudomonas aeruginosa, were the least frequent bacterial contaminants, their presence on environmental surfaces like ATMs is a cause for some alarm, because they have been shown to possess the potential to cause infection, especially in a hospital settings ^[8]. In different studies, each of these organisms has been implicated either as a major contaminant or as the most prevalent pathogenic bacteria recovered ^{[11, 29, 25]}.

Distribution of Isolates between the two major towns in Ebonyi State showed that a total of 14 bacterial isolates were obtained from the ATMs sampled, and out of the 14 isolates, 3 bacterial isolates representing 21.4% were obtained from ATM in Afikpo town, while 11 isolates representing 78.6% were from ATM from Abakaliki metropolis. The greater percentage of contaminations as observed among ATMs in Abakaliki in comparison to Afikpo was probably because of higher number of heterogeneous users in Abakaliki.

It has been observed that antibiotic susceptibility of bacterial isolates is not constant but dynamic and varies with time and environment ^[15]. There is the need for periodic screening of common bacterial pathogens for their antibiotic susceptibility profiles in different communities ^[27]. The antibiogram result of this study revealed that majority of the bacteria is highly resistant to standard antibiotics. Susceptibility of isolates ranges from 6.25% to 25.0% indicating that most of the antibiotics used were ineffective. S. aureus, Streptoccus spp and P. aeruginosa showed 68.75% resistance to the antibiotics, whilst E. coli (56.25%), Enterobacter spp (50%) and CNS showed 18.75% susceptibility each (Table 2). This result is similar to research undertaken on bacterial isolates on computer keyboards and mice in Cape Town, Ghana ^[31]. All Gram-positive and Gram-negative bacteria isolates showed 100% resistance to some of the common antibiotics in use. A. In a similar study, resistances (100%) of isolates to Ampicillin, Cloxacillin, Penicillin and Cefuroxime commonly used antibiotics has been observed in previous studies presenting a public health problem ^{[21, 32]}. Overall, Gentamicin was the most effective antibiotic, while Ofloxacin, Clindamycin, Cefixine, Levofloxacin and Ciprofloxacin were less effective.

5. Conclusion

This study of the bacterial contamination on ATM metallic keypads in Ebonyi state revealed the presence of pathogenic microbes like Staphylococcus aureus, Coagulase- negative Staphylococcus (CNS), Streptococcus species, Pseudomonas aeruginosa, Enterobacter species and Escherichia coli which were found to be resistant to some commonly used antibiotics. The result of this study is of public health concern as according to the result of this study ATM devices are potential areas for pathogen accumulation. Cleaning regimen aimed at reducing the population and presence of these organisms on the ATM surfaces should be developed using appropriate sanitizers and strictly adhered to by operators of such facilities. Further investigations are required to determine the status of ATMs according to fungi which were not studied in this investigation.

Acknowledgement

Researcher showed appreciation to management of the different banks who consented to taking part in the study by allowing us use their facilities. Funding for this study was from personal contributions from the authors, no external funding.

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