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From
Molecular characterization and Phylogenetic Analysis of
Clostridium botulinum
Mosaic Type D/C Isolated from Sudan
Dalia. A. Mohamed, Mohamed A. abdalla, Abdallah E. Ahmed, Mohamed M. Hassan, Abbas M. Ahmed, Mona O. Elhaj
American Journal of Microbiological Research
.
2017
, 5(3), 59-65 doi:10.12691/ajmr-5-3-2
Figure
1
.
Amplification of
C. botulinum
type C strain 2300 with primer set pM and pC, (L) molecular size standards (100 bp DNA ladder; Vivantis)
Full size figure and legend
Figure 2.
Amplification of sample 1 and 2 with primer set pM, pD and pC. (L) molecular size standards (100 bp DNA ladder; Vivantis)
Full size figure and legend
Figure 3.
(A)
Sequence chromatogram of the amplified segments produced with primer pC shown by Finch TV software, (B) BioEdit multiple sequence alignment shows the homology and evolutionary relation between Sudanese isolates (the amplified segments produced with primer pC) and reference strains. Differences were illustrated by arrows
Full size figure and legend
Figure
4
. Dendrogram showing the genetic relationships of
C.botulinum
isolate in Sudan (the amplified segments produced with primer pC) and different controls retrieved from database. The phylogram shows that sequence set pC for the two isolates are the closest
Full size figure and legend
Figure
5
.
(A)
Sequence chromatogram of the amplified segments produced with primer pD shown by Finch TV software, (B) BioEdit multiple sequence alignment shows the homology and evolutionary relation between Sudanese isolates (the amplified segments produced with primer pD) and reference strains. Differences were illustrated by arrows
Full size figure and legend
Figure
6
.
Dendrogram showing the genetic relationships of
C.botulinum
isolate in Sudan (the amplified segments produced with primer pD) and different controls retrieved from database. The phylogram shows that sequence set pD and BoNT/DC are the closest
Full size figure and legend
Figure
7
.
(A1) &
(A1)
Sequence chromatogram of the amplified segments produced with primer pM shown by Finch TV software, (B1)& (B1) BioEdit multiple sequence alignment shows the homology and evolutionary relation between Sudanese isolates (the amplified segments produced with primer pM) and reference strains
Full size figure and legend
Figure
8.
Dendrogram showing the genetic relationships of
C.botulinum
isolate in Sudan (the amplified segments produced with primer pM) and different controls retrieved from database. The phylogram shows that sequence set pM and BoNT/DC are the closest
Full size figure and legend