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From
Molecular Study of Panton-Valentine Leukocidin Genes among
Staphylococcus
aureus
Clinical Isolates in Khartoum State, Sudan
Najem Aldin M. Osman, Intisar E. Alrayah, Yassir Mahgoub Mohamed, Ali M El-Eragi, Muataz M. Eldirdery, Mohamed Ahmed Salih
American Journal of Microbiological Research
.
2015
, 3(3), 107-111 doi:10.12691/ajmr-3-3-2
Figure 1
.
A. Molecular detection of PVL virulence gene by PCR analyzed in 1.5% agarose gel electrophoresis
M: 100 bp molecular ladder, lane 1: positive control, lane 15: negative control, lane 2, 3, 4, 5, 6, 8, 11, 12, and 13 typical PVL positive isolates, lane: 7, 9, 14 PVL negative isolates. B.
lukS-PV
gene sequence chromatogram as showed by FinchTV software, left: site of new detected mutation, right: synonymous nucleotide polymorphism. C. BioEdit multiple sequence alignment of the twelve isolates and other selected strains from data base
Full size figure and legend
Figure 2.
Amino acids multiple sequence alignment of
lukS-PV
gene showed the mutation residues (Aspartic acid) at position 204 for the twelve Sudanese strains. The alignment was performed using ClustalW sequence alignment
Full size figure and legend
Figure 3.
Phylogenetic tree of the mutant Sudan
LukS PV
gene and other PVL genes obtained from database
Full size figure and legend