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From

Antibodies to Echinococcus granulosus Egg Antigens in Hydatid Patients: Characterization by Immunoblot and Diagnostic Value

Wissal IRAQI HOUSSAINI

American Journal of Microbiological Research. 2015, 3(1), 33-40 doi:10.12691/ajmr-3-1-5
  • Figure 1. 13% SDS-PAGE pattern of E. granulosus EA, PA and HCF in non reducing conditions. The position of the molecular weight markers is displayed on the left (Lane 1: EA, Lane 2: PA, Lane 3: HCF)
  • Figure 2. IB reactivity of hyperimmume rabbit antisera to HCF, PA or EA with HCF, PA or EA. The position of the molecular weight markers is displayed on the left. (A: Hyperimmume rabbit antisera to HCF, B: Hyperimumme rabbit antisera to PA, C: Hyperimmune rabbit antisera to EA) (Lane 1: EA, Lane 2: PA., Lane 3: HCF)
  • Figure 3. IB reactivity of moAb specific to Ag 5 with EA, PA and HCF. The molecular weight of the two subunits revealed, is displayed on the right (Lane 1: EA, Lane 2: PA, Lane 3: HCF)
  • Figure 4. IB reactivity to EA of 20 hydatid patient sera. The position of the molecular weight markers is displayed on the left
  • Figure 5. IB reactivity to PA of 20 hydatid patient sera. The position of the molecular weight markers is displayed on the left
  • Figure 6. IB reactivity to HCF antigens of 20 hydatid patient sera. The position of the molecular weight markers is displayed on the left
  • Figure 7. Distribution of individual results obtained in ELISA to EA (a), PA (b) and HCF (c) in 20 sera from patients with CE, 13 sera from healthy controls and 42 sera from subjects with other diseases. Mean absorbance value of the 55 sera controls plus 2 SD was taken as cut off level for positivity (Horizontal line). (Group 1: Patients with CE (n=20); Group 2: Healthy controls (n=13); Group 3: Individuals with ancylostomiasis (n=8); Group 4: Individuals with ascariasis (n=6); Group 5: Persons with hymenolepiasis (n=3); Group 6: Persons with shistosomiasis (n=5); Group 7: Individuals with viral hepatitis B (n=10); Group 8: Individuals with visceral leishmaniasis (n=10))