Testing the Quality of Biofertilizer <i>I</i><i>lletrisoy</i>

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The specific growth rate (μ) of each Illetrisoy bacterialis also been determined(Table 2). It is known that there are two groups of bacteria, there are a medium growing bacterial(double time4-6hours) with a symbol ++(KDL-92 andKDL-182) and a fast growing bacterial (double time2-4hours) with a symbol+++(KDL-196 andKDL-176). The big difference in the generation time of each bacteria lisolates indicates a difference in the capabilities isolates of to reproduce them self through the binary fission. Therefore, how much the number of cells that will be used in the formulation of biological fertilizers should be adjusted to the time of cell harvest of each isolate, so that composition of microbe cells in the biological fertilizer formulation may guaranteed to be active on the exponential phase.

Table 2. Result of measurement of the specific growth rate bacterial of Illetrisoy component

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The study of the Rawa Pening peat soil characteristics shows that on the field condition have a pH value of 6.82 and a water content of 46.31%. Total microbial population of the field condition is 11.4 x 10⁵cfug^-1 of soil. This matter is different from peat soil from other areas, which are usually acidic ^[16]. The Rawa Pening peat soil has C-organic content of 3.10 g kg^-1 and an average N content is 0.27 g kg^-1 which can be considered to have a medium value dignity ^[17]. Ratio C/N showed that the capability of RawaPening peat soil as a good organic source for plant growth (the average of ratio C/N 11.48). Not surprisingly, at this point the RawaPening peat soil is marketed as compost, which is good to plant growth medium.

Table 3. Result of the RawaPening peat soil analysis after wind dried process

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P levels of Rawa Pening peat soil categorized were low (5-7 g kg^-1), after conversion multiplied by 0.44 ^[17]. K levels categorized is also quite high, so whenever if the Rawa Pening peat soil is used as a growing medium, the plant responses to K fertilization is not so apparent, because the content of K soil > 0.4 cmol kg^-1^[17]. CEC value indicates capabilities the soil in the provide nutrients. CEC value of Rawa Pening peat soil is classified as very high (> 40 c mol kg^-1), as it contains organic matter that is high enough anyway.

The composition of carrier material is used the Rawa Pening peat soil after wind dried and powdered charcoal with a ratio of 3:1 and sterilized beforehand. Of the mixture have a pH value of 7.58 and an average water content of 4.80%. After the sterilization process of material carrier is carried out to inject bacterial suspension with an end product of biofertilizer Illetrisoy listed in Table 4.

Table 4. Quality of Illetrisoy A and Illetrisoy B

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Total of microbe on the Rawa Pening peat soil is 11.4 x10⁵cfug^-1 of soil. Besides microbes are aerobic, the anaerobic microbe is also found. After experiencing of the wind dried process, the peat soil physical condition changed on the moisture content, with a change in pH toward neutral (Table 2). After the wind dried process, total microbe contained in Rawa Pening peat soil decreased to 13.7 x 10⁴cfug^-1 of soil. Total microbe population is categorized as moderate, considering the microbial content in the bio-fertilizers on average 10⁷-10⁹cfug^-1 of material. The microbial content needs to be disabled or turned off, so as not to affect the biological activity of microbe on constituent of biofertilizer.

The Rawa Pening peat soil is chosen, because it has a good moisture characteristics of indicated by the high water holding capacity as well as the greater loss of water required to change the potential ^[18]. The viability of bacteria on the peat soil carrier influenced by the organic matter in the form of insoluble particles such as carbohydrates, proteins, and organic acids contained in peat as a source of carbon and energy for microbe metabolic activity. The viability and stability of bacteria is also determined by capabilities peat to maintain water content, pH neutral peat and the ability the bacteria to utilize carbon sources and energy sources that exist on the peat and the survival strategies the bacteria by using mechanisms that are owned by the respective efficiency each type ^[19].

Figure 1. Biofertilizer of Illetrisoy on three packaging shapes

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The composition of Illetrisoy biological fertilizer has a pH value and the amount of bacterial populations such as Table 6. From this starting composition will followed its development every 10 days of observation to a decline in the quality of bio fertilizers in the different storage conditions (temperature of 4^o C in a refrigerator, room temperature at 27°C, and in the incubator temperature 40°C).

Table 6. pH value and total populations of bacteria at biofertilizer Illetrisoyin some packaging

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Figure 2 showed the number of bacterial populations on the Illetrisoy biological fertilizers during the storage, with a time of interval sampling 10 days. In fact there is the same trend in the decline in the number of bacteria cells during storage of on the variety of package (plastic bags, allum unium foil and plastic bottles), the average shows a decline starts on the sampling day 9-10 or 90-100 days of storage. Of the three conditions is shown that the storage on the refrigerator more resilient than those stored at room temperature and incubator. The condition of the refrigerator caused the bacteria cells will enter a period of dormancy better than on the other storage conditions. Differences in packaging not show significant a difference on the calculation of the amount of bacterial populations. Differences in packaging will affect the cost and the level of difficulty in the packaging process only.

The viability of bacteria is better to could maintain at water content material by 50-60 %. It is advisable to could make biological fertilizers Illetrisoy on the water content 50-60 %. From the observations on the bacteria population during storage of Illetrisoy bio fertilizer with different package, it can be suggested that the package with a plastic bags better to do because it is cheap, easily available and quite simple in handling and transportation. Storage period may performed on the refrigerator or a cool place (temperature 4°C) not more for 3 months.

Figure 2. Total of bacteria population at bio-fertilizer Illetrisoy during storage

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