Figure 1. Efficiency of TNC knockdown by siRNA at mRNA and protein levels. (A) Real time PCR analysis shows the relative expression of total TNC and specific isoforms in transfected MDA-MB-231 cells with total TNC and high MW TNC siRNA compared to scrambled siRNA. The significant difference between TNC expression and scrambled siRNA are indicated by asterisk (***=≤0.001). (B) Western blot analysis of the transfected MDA-MB-231 breast cancer cell lines in both cell lysate, and conditioned media. siRNAs targeting different exons are represented in cell lysates and conditioned media. Upper panel represents predominant TNC isoforms (TNC-L and TNC-S). Lower panel represents loading control (Vinculin)

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Tenascin-C (TNC) Promotes Breast Cancer Cell Invasion and Proliferation: Functional Effects of TNC Knockdown in Highly Invasive Breast Cancer Cell Lines

Ali S Alharth, Musaad A Alsuliman, Abo Baker I Alshomrani, Waleed A Alyami

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