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From
The Atypical Expression of Retinoic Acid Inducible GATA6 Protein in Placenta is a Convenient Biomarker for Newborn Health Assessment
Abel Kangnidé, Denis Akpovo, Callinice D. Capo-Chichi
American Journal of Medical and Biological Research
.
2020
, 8(1), 17-23 doi:10.12691/ajmbr-8-1-3
Figure
1
. Western blot with 10% polyacrylamide gel showing GATA6 protein expression pattern in the placenta samples. The two isoforms of GATA6 are observed in sample 5z, 6A, 5x, 2H, and 2F (the Long type and the Short type). Sample 6B shows the loss of GATA6 type L, 3H and 3Z show undetectable expression of GATA6 by western blot. As loading control α-actin band was used
Full size figure and legend
Figure
2
. Western blot with 10% polyacrylamide gel showing newborn (stillborn) placenta sample without GATA6
L
(in red). Furthermore, samples 2z, 3B and 1I have normal expression of GATA6
L
and GATA6
S
. Sample 1H and 1 K have low expression of GATA6l, while samples 1L, 1M, and 1N have an overexpression in GATA6. β-Actin is used as a protein loading control
Full size figure and legend
Figure
3.
Western blot with 12% polyacrylamide gel showing a variation of GATA6 profiles in newborns. Sample 4N has both L and S type of GATA6. Sample 5N and 4S have additional bands at the bottom, indicating the presence of polymorphisms. Sample 2Y (in red) with a loss GATA6
L
, is another placenta sample of a stillborn baby
Full size figure and legend
Figure
4.
Histogram showing the frequency of GATA6-L isoform expressions in maternal placenta and the relation to newborn health status. The absence of expression is linked to stillborn (red); the low expression is associated to cardiopathies or metabolic syndromes (blue), the normal expression is not linked to sick children (green) while the overexpression is associated to cardiac hypertrophy
Full size figure and legend