Figure 3. The Dependence of Turnover Number on (A) Mn (II) and (B) ABTS Concentration in the Presence of Monovalent and Divalent Ions. () MnSO₄ only_, () 0.8 mM NaSO₄, () 0.8 mM ZnSO₄, () 0.2 mM CuSO₄, () 0.8 mM CoSO₄. Reaction mixtures contained 100 mM Na tartrate buffer, pH 5.0 at 25°C and fixed H₂O₂ concentration (0.1 mM). Varying concentrations of MnSO₄ and ABTS were used for figures A and B respectively as stated in the material and methods section. Apparent kinetic parameters were estimated from the best fit to the Michaelis-Menten equation

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Role of Some Metal Ions on Steady–state Kinetics of Engineered Wild–type and Manganese (II) Binding Site Mutants of Recombinant Phlebia radiata Manganese Peroxidase 3 (rPr-MnP3)

Usenobong F. Ufot, Aniefiok E. Ite, Idorenyin H. Usoh, Monday I. Akpanabiatu

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