Figure 2. Electrophoretic typical gel analysis for the effect of LPS/SB203580 on IκB-α cytosolic phosphorylation. (A) Cell cultures were pretreated with ascending concentrations of SB203580 for 1h, followed by incubation with LPS for 24h. The phosphorylated (pIκB-α) and non-phosphorylated forms of IκB-α were subsequently determined. Semi-quantitative loading per lane is verified by the consistent expression of the constitutive form of β-actin (B) Histogram analysis of the relative levels of pIκB-α and IκB-α with selective MAPK^p38 inhibition. The number of experimental observations is n = 3 – 5, for separate and independently prepared cell cultures of alveolar epithelial cells with or without pre-treatments with LPS/SB203580

From

The Irreversible Inhibition of the MAPK^p38 Pathway Downregulates LPS-augmented Release of Interleukin-Related Inflammatory Cytokines (IL-1β, IL-6): Immune Surveillance Unraveling IκB-α/NF-κB Phosphorylation State-independent Mechanism in vitro

John J Haddad

• Previous
• Figure 2 of 3  (Figures index)
• Next