Figures index

From

Effects of Tenascin-C (TNC) Knockdown on Global Genes Expression

Ali S. Alharth, Sherien M. El-Daly

American Journal of Medical and Biological Research. 2015, 3(2), 62-67 doi:10.12691/ajmbr-3-2-3
  • Figure 1. Relative expression (RE) means and (± SEM) of TNC between samples in breast cancer cell lines transfected with total TNC siRNA at 24 and 48 hrs post transfections compared to scrambled siRNA. All results are highly significant as indicated by the stars (***=p≤0.001).
  • Figure 2. Hierarchical clustering of samples identifies two main clusters, representing the two different cell lines. Within each cluster, samples were again divided by siRNA treatment and time point. Red, positive log2 ratio; Green, negative log2 ratio
  • Figure 3. Box plot of all samples pre and post normalisation. Each sample represented by one box plot. The blue box plots represent log2 ratio for raw intensity values, and the red box plots represent log2 ratio for normalised data
  • Figure 4. Venn diagram reveals overlapping genes amongst breast cancer cell lines that are differentially regulated as a result of total TNC knockdown in MDA-MB-436 and -231 cells at 24 and 48 hrs.
  • Figure 5. Relative quantitation (RQ) values of regulated genes in transfected MDA-MB-231 and MDA-MB-436 breast cancer cell line with total TNC siRNA compared to scrambled siRNA control. The graph shows the mean RQ value (± SEM) between samples compared to scrambled siRNA. Significant differences (** = p≤0.01; ***=p≤0.001) are indicated by asterisks