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Comparative Study of Some Potential Paracrine Factors Produced by Normal and Androgenetic Alopecia Hair Follicles

Saeed A. Alwaleedi

American Journal of Medical and Biological Research. 2015, 3(1), 38-47 doi:10.12691/ajmbr-3-1-3
  • Figure 1. Electrophoretic separation of total RNA. RNA from balding samples (n=3) and non-balding samples (n=3) were evaluated using the Agilent 2100 Bioanalyzer. The rRNA bands (28S and 18S) exhibited 2:1 ratio and are clearly visible in the total RNA gels
  • Figure 2. Electropherogram of total RNA samples. RNA obtained from balding (n=3) and normal samples (n=3) were evaluated using the Agilent Bioanalyzer. The graphs show high quality RNA due to presence of clear 28S and 18S peaks, and the noise between the peaks is very low, with minimal low molecular weight noise
  • Figure 3. Scatter plot shows the correlation of gene expression between balding (n=3) and non-balding follicles (n=3). The correlation curve shows that gene expression follows a downward slope indicating that the pattern of gene expression is running in a band from upper left to lower right. The correlation coefficient (r) equals -0.74 indicating there is a negative correlation (P<0.05) in gene expression between the balding group and non-balding one
  • Figure 4. Principal components analysis (PCA) shows the discrimination of balding and non-balding samples. Three-dimensional graph, PCA analysis, shows that the experimental samples separated into two distinct groups, balding (blue dots) and non-balding (red dots). PC1, PC2 and PC3 are plotted on the x, y and z axes respectively. PC1 and PC2 indicate 20.68% and 17.17% variation respectively within the same group, whereas PC3 indicates 55.36% variation in the total gene expression
  • Figure 5. A comparison of the gene expression profile in individual balding and non-balding follicle samples from androgenetic alopecia men using a microarray profiling approach. Hierarchical clustering diagram showing individual expression patterns for balding samples (2, 4, 6) versus non-balding ones (1, 3, 5). The diagram shows clear differences in gene expression between the two conditions in each individual
  • Figure 6. Comparison of the expression levels for some potential paracrine factor genes (A), Keratin genes (B), and keratin-related protein genes (C) in normal (blue) and androgenetic alopecia (brown) hair follicles. The bar diagrams show the quantitative real-time PCR expression pattern of the selected genes from microarray data. Gene expression of individual samples was normalized to GAPDH. The overall validation data show the same trend obtained from microarray analysis. Data are the mean values ± SEM from three different individuals in both conditions. (*P<0.05, **P<0.01, ***P<0.001)