Figure 3. Western blot analysis in conditioned media of MDA-MB-231 breast cancer cell lines transfected with siRNA targeting high MW TNC isoforms, total TNC and scrambled siRNA. A) H300 anti-TNC antibody reactivity confirmed the down-regulated expression of total TNC and high MW TNC isoforms. B) Anti- TSP-1 reactivity showed the up-regulated expression of TSP-1 in cells transfected with total TNC and high MW TNC isoforms siRNAs. C) Loading control (Vinculin)

From

Investigation of Tenascin-C Effects on Thrombospondin 1 in Highly Invasive Breast Cancer Cell Lines

Ali Saleh Alharth, Musaad Abdullah Alsuliman, Waleed Ahmed Alyami, Abobaker Alshamarni, Zyad M Alsulaiman, Abdulaziz M Alsulaiman

American Journal of Medical and Biological Research. 2024, 12(2), 81-84 doi:10.12691/ajmbr-12-2-7