Figure 3. Inhibition of p21 decreased breast cancer cells’ clonogenic and sphere formation abilities. (A) Colony formation photos and quantification of CFU of MDA-MB-231 shCtrl cells and MDA-MB-231 shp21 cells. (B) Sphere formation photos and quantification of the sphere formation efficiency of MDA-MB-231 shCtrl and MDA-MB-231shp21 cells. (C) Relative mRNA levels of stem-related genes CD90, CD133, OCT4, and NANOG were decreased in MDA-MB-231 shp21 cells. (D) Colony formation photos and quantification of CFU of MCF-7 shCtrl cells and MCF-7 shp21 cells. (E) Sphere formation photos and quantification of the sphere formation efficiency of MCF-7 shCtrl cells and MCF-7 shp21 cells. Student’s t-test was used to compare the two groups. Data are shown as the mean ± standard deviation. shCtrl = short hairpin RNA control; shp21 = short hairpin RNA targeting p21; CFU = colony-forming units. : Breast Cancer Cells and Impairs the Tumor-Killing Effect of the Vaccinia Virus : Science and Education Publishing

Figure 3. Inhibition of p21 decreased breast cancer cells’ clonogenic and sphere formation abilities. (A) Colony formation photos and quantification of CFU of MDA-MB-231 shCtrl cells and MDA-MB-231 shp21 cells. (B) Sphere formation photos and quantification of the sphere formation efficiency of MDA-MB-231 shCtrl and MDA-MB-231shp21 cells. (C) Relative mRNA levels of stem-related genes CD90, CD133, OCT4, and NANOG were decreased in MDA-MB-231 shp21 cells. (D) Colony formation photos and quantification of CFU of MCF-7 shCtrl cells and MCF-7 shp21 cells. (E) Sphere formation photos and quantification of the sphere formation efficiency of MCF-7 shCtrl cells and MCF-7 shp21 cells. Student’s t-test was used to compare the two groups. Data are shown as the mean ± standard deviation. shCtrl = short hairpin RNA control; shp21 = short hairpin RNA targeting p21; CFU = colony-forming units.

American Journal of Medical and Biological Research. 2024, 12(2), 36-44 doi:10.12691/ajmbr-12-2-1