Figures index

From

Identification of Novel Multi Epitopes Vaccine against the Capsid Protein (ORF2) of Hepatitis E Virus

Mashair A. A. Nouri, Yassir A. Almofti, Khoubieb Ali Abd-elrahman, Elsideeq E. M. Eltilib

American Journal of Infectious Diseases and Microbiology. 2019, 7(1), 26-42 doi:10.12691/ajidm-7-1-5
  • Figure 1. Evolutionary divergence analysis of capsid protein of the HEV. The retrieved strains showed divergence in their common ancestors
  • Figure 2. Multiple sequence alignment (MSA) of the retrieved strains using Bioedit software and ClustalW. Dots indicated the conservancy and letters in cubes showed the alteration in amino acid
  • Figure 3. Prediction of B-cell epitopes using (a) Bepipred linear epitope, threshold value 0.353 (b) Emini surface accessibility, threshold value 1.000 and (c) Kolaskar & Tongaonkar antigenicity methods, threshold value 1.031. Yellow areas above the threshold (red line) are suggested to be a part of B cell epitope, while green areas are not
  • Figure 4. Position of proposed conserved B cell epitopes in structural level of the capsid protein. Five epitopes were illustrated in this figure to interact with B cell. These epitopes showed conservancy, high score in surface accessibility and antigenicity using IEDB software. The ball like structures represent the predicted epitopes. The position of these epitopes was according to their position in the capsid protein
  • Figure 5. T cell proposed epitopes that interact with MHC-I alleles. These epitopes are 367GIALTLFNL375, 379LLGGLPTEL387, 394QLFYSRPVV402 and 389SSAGGQLFY397. The first three epitopes were found interacting with both MHC-1 and MHC-II alleles. The latter interacted only with MHC-I alleles and it was shown in figure (6). The ball like structures represent the predicted epitopes. The positions of these proposed epitopes was according to their position in the capsid protein of HEV
  • Figure 6. T cell proposed epitopes that interact with MHC-II alleles. These epitopes were 205YAISISFWP213, 299LLDFALELE307, 341LTTTAATRF349, 368IALTLFNLA376, 367GIALTLFNL375, 379LLGGLPTEL387 and 394QLFYSRPVV402. The last three epitopes were shown in Figure 5. The epitope 389SSAGGQLFY397 also interacted with MHC-1 alleles. The ball like structures represent the predicted epitopes. The positions of these proposed epitopes was according to their position in the capsid protein of HEV