Figure 4. Effects of hydrogenized water on SIRT1 expression in human umbilical vein endothelial cells. Human umbilical vein endothelial cells were incubated with hydrogenized water at 0, 3.125, 6.25, 12.5, 25 and 50% v/v for 24 hour. Cell were treated with concentrations of 100 μM Resveratrol for 24 hr as a control. Cells were lysed and lysates prepared for SIRT1 and GAPDH Western blotting

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Effects of Hydrogenized Water on Intracellular Biomarkers for Antioxidants, Glucose Uptake, Insulin Signaling and SIRT 1 and Telomerase Activity

Robert Settineri, Jin Ji, Chunlan Luo, Rita R. Ellithorpe, Gonzalo Ferreira de Mattos, Steven Rosenblatt, James LaValle, Antonio Jinenez, Shigeo Ohta, Garth L. Nicolson

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